Hisajima T, Ishibashi H, Yamada T, Nishiyama Y, Yamaguchi H, Funakoshi K, Abe S
Teikyo University Institute of Medical Mycology, Tokyo.
Med Mycol. 2008 Nov;46(7):697-704. doi: 10.1080/13693780802039919.
We analyzed the morphologic and microbiologic aspects of the process of adhesion and invasion in the early stages of Candida albicans oral infection in a murine system. ICR mice were anesthetized by intramuscular injection with chlorpromazine chloride and then orally inoculated by swabbing with the C. albicans yeast cells. Their tongues were resected 1-3h after inoculation, washed sequentially with a physiological saline and 0.25% trypsin-solution and then homogenized. The number of viable C. albicans cells on the tongue surface was counted and fround to increase from 1-3h after inoculation. Most of the Candida cells attached to the tongue surface were present in clusters, mainly located in the gaps between lingual papillae and were covered with a mucoidal substance. By 3h after inoculation, these clusters frequently formed mycelia and could not be easily detached from the tongue surface by trypsin treatment. Observation of SEM and histological sections stained by Fungiflora Y revealed that the Candida hyphae at 3h stretched out of the cluster and entered the tongues through the surface. These results indicate that Candida hyphae begin to invade the tongue surface within 3h after inoculation and suggest that the mucus-like substance covering these cells may have an important early role in the interaction between the Candida cells and the tongue mucosal epithelium.
我们在小鼠系统中分析了白色念珠菌口腔感染早期黏附与侵袭过程的形态学和微生物学方面。通过肌肉注射氯丙嗪对ICR小鼠进行麻醉，然后用白色念珠菌酵母细胞擦拭口腔进行接种。接种后1 - 3小时切除它们的舌头，依次用生理盐水和0.25%胰蛋白酶溶液冲洗，然后匀浆。计数舌表面存活的白色念珠菌细胞数量，发现接种后1 - 3小时数量增加。大多数附着在舌表面的念珠菌细胞呈簇状存在，主要位于舌乳头之间的间隙中，并被一种黏液物质覆盖。接种后3小时，这些簇状物频繁形成菌丝体，经胰蛋白酶处理后不易从舌表面分离。扫描电子显微镜观察和用Fungiflora Y染色的组织切片显示，3小时时念珠菌菌丝从簇状物中伸出并通过表面进入舌头。这些结果表明，念珠菌菌丝在接种后3小时内开始侵入舌表面，并表明覆盖这些细胞的黏液样物质可能在念珠菌细胞与舌黏膜上皮之间的相互作用中起重要的早期作用。
