Mima Ewerton Garcia de Oliveira, Pavarina Ana Cláudia, Dovigo Lívia Nordi, Vergani Carlos Eduardo, Costa Carlos Alberto de Souza, Kurachi Cristina, Bagnato Vanderlei Salvador
Dentistry School, São Francisco University-USF, Bragança Paulista, SP, Brazil.
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2010 Mar;109(3):392-401. doi: 10.1016/j.tripleo.2009.10.006. Epub 2010 Jan 8.
In vivo studies of antimicrobial PDT in animal models of oral candidosis are scarce and the association of porphyrin and LED light has not been evaluated for in vivo photoinactivation of Candida. In this study the effectiveness of photodynamic therapy (PDT) on the inactivation of Candida albicans in vivo was evaluated.
Seventy-one 6-week-old female Swiss mice were immunosuppressed, provided tetracycline to their drinking water, then orally swabbed with a suspension of C. albicans (10(7) CFU/mL). Four days after oral inoculation, PDT was performed on the dorsum of the tongue after topical administration of Photogem at 400, 500, or 1000 mg/L and followed 30 minutes later by illumination with LED light (305 J/cm(2)) at 455 or 630 nm (n = 5 each). After swabbing to recover yeast from the tongue, the number of surviving yeast cells was determined (CFU/mL) and analyzed by ANOVA and Holm-Sidak tests (P < .05). Animals were humanely killed, and the tongues surgically removed and processed for histological evaluation of presence of yeast and inflammatory reaction.
PDT resulted in a significant reduction in C. albicans recovered from the tongue (P < .001) when compared with mice from the positive control group. There was no difference between the concentrations of Photogem and LED light wavelengths used. Histological evaluation of the tongue revealed that PDT causes no significant adverse effects to the local mucosa.
PDT promoted significant reduction in the viability of C. albicans biofilm without harming the tongue tissue.
关于口腔念珠菌病动物模型中抗菌光动力疗法（PDT）的体内研究较少，且尚未评估卟啉与发光二极管（LED）光联合用于念珠菌体内光灭活的效果。本研究评估了光动力疗法（PDT）对体内白色念珠菌灭活的有效性。
71只6周龄雌性瑞士小鼠接受免疫抑制处理，饮用含四环素的水，然后用白色念珠菌悬液（10⁷CFU/mL）进行口腔擦拭接种。口腔接种4天后，在舌背局部给予浓度为400、500或1000mg/L的光姆（Photogem），30分钟后分别用波长为455或630nm的LED光（305J/cm²）照射（每组n = 5只），进行PDT治疗。擦拭舌部以回收酵母后，测定存活酵母细胞数（CFU/mL），并通过方差分析和霍尔姆 - 西达克检验进行分析（P < 0.05）。对动物实施安乐死，手术切除舌头并进行处理，以对酵母的存在和炎症反应进行组织学评估。
与阳性对照组小鼠相比，PDT治疗后从舌部回收的白色念珠菌数量显著减少（P < 0.001）。所用光姆浓度和LED光波长之间无差异。舌部组织学评估显示，PDT对局部黏膜无显著不良影响。
PDT可显著降低白色念珠菌生物膜的活力，且不损害舌组织。
