Scarberry Kenneth E, Dickerson Erin B, McDonald John F, Zhang Z John
School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, Georgia 30332, USA.
J Am Chem Soc. 2008 Aug 6;130(31):10258-62. doi: 10.1021/ja801969b. Epub 2008 Jul 9.
Magnetic cobalt spinel ferrite nanoparticles coated with biocompatible polygalacturonic acid were functionalized with ligands specific for targeting expressed EphA2 receptors on ovarian cancer cells. By using such magnetic nanoparticle-peptide conjugates, targeting and extraction of malignant cells were achieved with a magnetic field. Targeting ovarian cancer cells with receptor specific peptide-modified magnetic nanoparticles resulted in cell capture from a flow stream in vitro and from the peritoneal cavity of mice in vivo. Successful removal of metastatic cancer cells from the abdominal cavity and circulation using magnetic nanoparticle conjugates indicate the feasibility of a dialysis-like treatment and may improve long-term survival rates of ovarian cancer patients. This approach can be applied for fighting other cancers, such as leukemia, once the receptors on malignant cells are identified and the efficacy of targeting ligands is established.
涂覆有生物相容性聚半乳糖醛酸的磁性钴尖晶石铁氧体纳米颗粒用特异性靶向卵巢癌细胞上表达的EphA2受体的配体进行功能化。通过使用这种磁性纳米颗粒-肽缀合物，利用磁场实现了恶性细胞的靶向和提取。用受体特异性肽修饰的磁性纳米颗粒靶向卵巢癌细胞导致在体外从流动流中以及在体内从小鼠腹腔中捕获细胞。使用磁性纳米颗粒缀合物成功地从腹腔和循环中去除转移性癌细胞表明了类似透析治疗的可行性，并可能提高卵巢癌患者的长期生存率。一旦确定了恶性细胞上的受体并建立了靶向配体的功效，这种方法可用于对抗其他癌症，如白血病。
