Sun Xuefei, Liu Jikun, Lee Milton L
Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT 84602, USA.
Electrophoresis. 2008 Jul;29(13):2760-7. doi: 10.1002/elps.200800005.
In-channel atom transfer radical polymerization (ATRP) was used to graft a PEG layer on the surface of microchannels formed in poly(glycidyl methacrylate)-co-(methyl methacrylate) (PGMAMMA) microfluidic devices. The patterned and cover plates were first anchored with ATRP initiator and then thermally bonded together, followed by pumping a solution containing monomer, catalyst, and ligand into the channel to perform ATRP. A PEG-functionalized layer was grafted on the microchannel wall, which resists protein adsorption. X-ray photoelectron spectroscopy (XPS) was used to investigate the initiator-bound surface, and EOF was measured to evaluate the PEG-grafted PGMAMMA microchannel. Fast, efficient, and reproducible separations of amino acids, peptides, and proteins were obtained using the resultant microdevices. Separation efficiencies were higher than 1.0x10(4) plates for a 3.5 cm separation microchannel. Compared with microdevices modified using a previously reported ATRP technique, these in-channel modified microdevices demonstrated better long-term stability.
采用通道内原子转移自由基聚合（ATRP）法在聚（甲基丙烯酸缩水甘油酯）-共-（甲基丙烯酸甲酯）（PGMAMMA）微流控器件中形成的微通道表面接枝聚乙二醇（PEG）层。首先将带有图案的板和盖板用ATRP引发剂固定，然后热键合在一起，接着将含有单体、催化剂和配体的溶液泵入通道中进行ATRP反应。在微通道壁上接枝了具有抗蛋白质吸附功能的PEG功能化层。利用X射线光电子能谱（XPS）对引发剂结合的表面进行了研究，并通过测量电渗流（EOF）对PEG接枝的PGMAMMA微通道进行了评估。使用所得的微器件实现了对氨基酸、肽和蛋白质的快速、高效且可重复的分离。对于3.5 cm长的分离微通道，分离效率高于1.0×10⁴理论塔板数。与使用先前报道的ATRP技术修饰的微器件相比，这些通道内修饰的微器件表现出更好的长期稳定性。
