Liu Jikun, Sun Xuefei, Lee Milton L
Department of Chemistry and Biochemistry, Brigham Young University, Provo, Utah 84602-5700, USA.
Anal Chem. 2005 Oct 1;77(19):6280-7. doi: 10.1021/ac0580060.
A surface-reactive acrylic polymer, poly(glycidyl methacrylate-co-methyl methacrylate) (PGMAMMA), was synthesized and evaluated for suitability as a substrate for fabrication of microfluidic devices for chemical analysis. This polymer has good thermal and optical properties and is mechanically robust for cutting and hot embossing. A key advantage of this polymeric material is that the surface can be easily modified to control inertness and electroosmotic flow using a variety of chemical procedures. In this work, the procedures for aminolysis, photografting of linear polyacrylamide, and atom-transfer radical polymerization on microchannel surfaces in PGMAMMA substrates were developed, and the performance of resultant microfluidic electrophoresis devices was demonstrated for the separation of amino acids, peptides, and proteins. Separation efficiencies as high as 4.6 x 10(4) plates for a 3.5-cm-long separation channel were obtained. The results indicate that PGMAMMA is an excellent substrate for microfabricated fluidic devices, and a broad range of applications should be possible.
合成了一种表面反应性丙烯酸聚合物聚（甲基丙烯酸缩水甘油酯 - 甲基丙烯酸甲酯）（PGMAMMA），并评估了其作为制造用于化学分析的微流控装置的基底的适用性。这种聚合物具有良好的热性能和光学性能，并且在切割和热压花方面具有机械强度。这种聚合物材料的一个关键优势是，可以使用各种化学方法轻松修饰其表面，以控制惰性和电渗流。在这项工作中，开发了在PGMAMMA基底的微通道表面上进行氨解、线性聚丙烯酰胺的光接枝和原子转移自由基聚合的方法，并展示了所得微流控电泳装置用于分离氨基酸、肽和蛋白质的性能。对于3.5厘米长的分离通道，获得了高达4.6×10⁴理论塔板数的分离效率。结果表明，PGMAMMA是用于微制造流体装置的优良基底，并且应该有广泛的应用可能性。
