Cavallaro J F, Kemp P D, Kraus K H
Department of Matrix Engineering, Organogenesis Inc, 150 Dan Road, Canton, Massachusetts 02021, USA.
Biotechnol Bioeng. 1994 Apr 5;43(8):781-91. doi: 10.1002/bit.260430813.
Tissue-engineered implants require appropriate biomaterials to serve the required physical function of the tissue being repaired or replaced while facilitating remodeling of the implant. We report on the development of implantable fabrics manufactured from continuous collagen threads. The collagen threads are formed by extrusion of native, acid-extracted bovine collagen into a buffered solution of polyethylene glycol, followed by rinsing and air drying. The high manufacturing rate of such threads permits the production of collagen fabrics of various configurations. The fiber diameter can be controlled, and threads with dry diameters as low as 25 microm have been produced. Braids and bundles of collagen threads implanted as a replacement of the anterior cruciate ligament in a dog model were completely remodeled into host tissue by 12 weeks. Knitted collagen fabrics implanted in a rat abdominal repair model prevented herniation, and connective tissue ingrowth was observed within the fabric by 12 weeks.
组织工程植入物需要合适的生物材料来发挥所修复或替代组织所需的物理功能，同时促进植入物的重塑。我们报告了由连续胶原纤维制成的可植入织物的研发情况。胶原纤维是通过将天然酸提取牛胶原挤压到聚乙二醇缓冲溶液中形成的，随后进行冲洗和风干。这种纤维的高制造速率使得能够生产各种结构的胶原织物。纤维直径可以控制，并且已经生产出干直径低至25微米的纤维。在狗模型中作为前交叉韧带替代品植入的胶原纤维编织物和束在12周时完全重塑为宿主组织。植入大鼠腹部修复模型中的针织胶原织物可防止疝形成，并且在12周时观察到织物内有结缔组织长入。
