Laboratory of Blue Biotechnology & Aquatic Bioproducts, Institut National des Sciences et Technologies de la Mer (INSTM), INSTM, 28, rue 2 mars 1934, Salammbô, Tunis 2025, Tunisia.
Institut National Agronomique de Tunisie (INAT), Université de Carthage, Tunis 1082, Tunisia.
Mar Drugs. 2018 Jun 15;16(6):211. doi: 10.3390/md16060211.
The acid solubilised collagen (ASC) and pepsin solubilised collagen (PSC) were extracted from the by-products (skin) of a cartilaginous fish (). The ASC and PSC yields were 23.07% and 35.27% dry weight, respectively and were identified as collagen Type I with the presence of α, β and γ chains. As revealed by the Fourier Transform Infrared (FTIR) spectra analysis, pepsin did not alter the PSC triple helix structure. Based on the various type of collagen yield, only PSC was used in combination with chitosan to produce a composite film. Such film had lower tensile strength but higher elongation at break when compared to chitosan film; and lower water solubility and lightness when compared to collagen film. Equally, FTIR spectra analysis of film composite showed the occurrence of collagen-chitosan interaction resulting in a modification of the secondary structure of collagen. Collagen-chitosan-based biofilm showed a potential UV barrier properties and antioxidant activity, which might be used as green bioactive films to preserve nutraceutical products.
酸溶性胶原蛋白（ASC）和胃蛋白酶溶性胶原蛋白（PSC）是从软骨鱼（）的副产品（皮肤）中提取的。ASC 和 PSC 的产率分别为 23.07%和 35.27%干重，并且被鉴定为存在 α、β 和 γ 链的 I 型胶原蛋白。傅里叶变换红外（FTIR）光谱分析表明，胃蛋白酶未改变 PSC 的三螺旋结构。根据各种类型胶原蛋白的产率，仅使用 PSC 与壳聚糖结合生产复合膜。与壳聚糖膜相比，该膜的拉伸强度较低，但断裂伸长率较高；与胶原蛋白膜相比，其水溶性和亮度较低。同样，膜复合材料的 FTIR 光谱分析表明发生了胶原蛋白-壳聚糖相互作用，导致胶原蛋白二级结构的修饰。基于胶原蛋白-壳聚糖的生物膜具有潜在的紫外线阻隔性能和抗氧化活性，可作为绿色生物活性膜用于保存营养保健品。
