Nahkuri Satu, Taft Ryan J, Korbie Darren J, Mattick John S
Institute for Molecular Bioscience, University of Queensland, St. Lucia, Queensland 4072, Australia.
J Mol Biol. 2008 Sep 12;381(4):810-5. doi: 10.1016/j.jmb.2008.06.057. Epub 2008 Jun 28.
HBII-52 is a human brain-specific C/D box snoRNA that potentially regulates the editing and/or alternative splicing of the serotonin receptor. Forty-two nearly identical copies of the HBII-52 gene are located immediately downstream of the SNRPN protein-coding gene in an imprinted locus associated with Prader-Willi syndrome. Other eutherian mammals, with genomic assemblies covering the corresponding locus, also have multiple orthologous copies of HBII-52. The SNRPB gene, which is known to have given rise to SNRPN through gene duplication, expresses a C/D box snoRNA, SNORD119, from its fifth intron. Here we show that, despite the fact that they lie in different positions relative to the orthologous SNRPB/SNRPN coding sequences, there are significant sequence similarities between SNORD119 and HBII-52, including the antisense element and the stem-forming regions. By analysing these snoRNAs in marsupial and eutherian mammal genomes, we reconstruct the likely evolutionary history of the HBII-52 cluster and SNORD119 and suggest that they have evolved from a common ancestor.
HBII-52是一种人类大脑特异性C/D盒小核仁RNA（snoRNA），可能调控血清素受体的编辑和/或可变剪接。HBII-52基因的42个几乎相同的拷贝位于与普拉德-威利综合征相关的印记基因座中SNRPN蛋白质编码基因的紧邻下游。其他有覆盖相应基因座的基因组组装的真兽类哺乳动物也有多个HBII-52的直系同源拷贝。已知通过基因复制产生SNRPN的SNRPB基因从其第五个内含子表达一种C/D盒小核仁RNA，即SNORD119。我们在此表明，尽管SNORD119和HBII-52相对于直系同源的SNRPB/SNRPN编码序列处于不同位置，但它们之间存在显著的序列相似性，包括反义元件和茎形成区域。通过分析有袋类和真兽类哺乳动物基因组中的这些小核仁RNA，我们重建了HBII-52基因簇和SNORD119可能的进化历史，并表明它们是从一个共同祖先进化而来的。
