Zhang Linlin, Dong Shuqing, Yang Zhiyong, Wang Qingjiang, He Pingang, Fang Yuzhi
Department of Chemistry, East China Normal University, Shanghai 200062, China.
J Pharm Biomed Anal. 2008 Sep 10;48(1):198-200. doi: 10.1016/j.jpba.2008.04.017. Epub 2008 Apr 25.
The simultaneous determination of four active ingredients in Nao Xue Shuan Tablets, including ferulic acid, protocatechuic aldehyde, caffeic acid and protocatechuic acid was performed by capillary zone electrophoresis with amperometric detection (CZE-AD). The effects of working electrode potential, pH and concentration of running buffer, separation voltage and injection time on CZE-AD were investigated. Under the optimum conditions, the four analytes could be perfectly separated within 18 min. A 300 microm diameter carbon-disc electrode had a good response at +0.95 V (vs. SCE) for the four analytes. The response was linear over three orders of magnitude with detection limits (S/N=3) as low as 10(-8) or 10(-9) g/mL for the analytes. The assay results were satisfactory with recoveries in the range of 85.2-93.0% and RSDs less than 3.3%.
采用毛细管区带电泳安培检测法（CZE-AD）同时测定脑血酸片中阿魏酸、原儿茶醛、咖啡酸和原儿茶酸四种活性成分。考察了工作电极电位、pH值、运行缓冲液浓度、分离电压和进样时间对CZE-AD的影响。在最佳条件下，四种分析物可在18分钟内完美分离。直径为300微米的碳盘电极对四种分析物在+0.95 V（相对于SCE）时具有良好的响应。响应在三个数量级上呈线性，分析物的检测限（S/N=3）低至10^(-8)或10^(-9) g/mL。测定结果令人满意，回收率在85.2-93.0%范围内，相对标准偏差小于3.3%。
