Maruyama Tatsuo, Yamamura Hiroshi, Hiraki Mai, Kemori Yoshinori, Takata Harumi, Goto Masahiro
Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, Fukuoka 819-0395, Japan.
Colloids Surf B Biointerfaces. 2008 Oct 1;66(1):119-24. doi: 10.1016/j.colsurfb.2008.05.017. Epub 2008 Jun 4.
We investigated DNA-directed aggregation of vesicles using DNA-surfactants. Following tethering of single-stranded DNA oligonucleotides to vesicles using DNA-surfactant, the tethered vesicles were assembled with other vesicles bearing complementary strands. The vesicle aggregation was strongly affected by the salt concentration and by temperature according to the characteristics of DNA hybridization. Restriction enzyme, which can hydrolyze the double-stranded DNA used in the present study, dissociated the vesicle aggregates. Exploration using fluorescently labeled vesicles suggested that the DNA-directed vesicle aggregation took place in a sequence-specific manner through DNA-duplex formation. Interestingly, the DNA-directed aggregation using short DNA-surfactant induced the fusion of vesicles to produce giant vesicles, resulting in an enzymatic reaction in the giant vesicle.
我们使用DNA表面活性剂研究了囊泡的DNA定向聚集。在使用DNA表面活性剂将单链DNA寡核苷酸连接到囊泡上之后，连接的囊泡与带有互补链的其他囊泡组装在一起。根据DNA杂交的特性，盐浓度和温度对囊泡聚集有强烈影响。能够水解本研究中使用的双链DNA的限制酶使囊泡聚集体解离。使用荧光标记囊泡的探索表明，DNA定向囊泡聚集通过DNA双链形成以序列特异性方式发生。有趣的是，使用短DNA表面活性剂的DNA定向聚集诱导囊泡融合以产生巨型囊泡，从而在巨型囊泡中引发酶促反应。
