Frengova G, Simova E, Pavlova K, Beshkova D, Grigorova D
Laboratory of Applied Microbiology, Institute of Microbiology, Bulgarian Academy of Sciences, 4000 Plovdiv, 26 Maritza Blvd., Plovdiv, Bulgaria.
Biotechnol Bioeng. 1994 Oct;44(8):888-94. doi: 10.1002/bit.260440804.
The growth and carotenoid biosynthesis of the yeast Rhodotorula glutinis was studied by cocultivation with Lactobacillus helveticus in cheese ultrafiltrate containing 3.9% and 7.1% lactose. By growing this mixed culture in a 15-L fermentor MBR AG (Switzerland) at an air flow rate of 0.5 L/L min and agitation at 220 rpm for 6 days, a total yield of carotenoids of 268 mug/g dry cells wasobtained. Carotenoids were formed almost parallel with the cell growth, anda maximum production was reached at an early stationary phase. A high-performance liquid chromatographic system (HPLC) permitting simultaneous determination of major carotenoid pigments was used. The three main pigments (torularhodin, beta-carotene, and torulene) were formed in Rhodotorula glutinis, and reached a maximum concentration as follows: 182.0, 43.9, 23.0 mug,g dry cells. (c) 1994 John Wiley & Sons, Inc.
通过在含有3.9%和7.1%乳糖的奶酪超滤物中与瑞士乳杆菌共培养，研究了粘红酵母的生长和类胡萝卜素生物合成。在瑞士MBR AG公司的15升发酵罐中，以0.5升/升·分钟的空气流速和220转/分钟的搅拌速度培养这种混合培养物6天，获得了类胡萝卜素总产量为268微克/克干细胞。类胡萝卜素的形成几乎与细胞生长同步，在早期稳定期达到最大产量。使用了一种能够同时测定主要类胡萝卜素色素的高效液相色谱系统（HPLC）。粘红酵母中形成了三种主要色素（圆酵母红素、β-胡萝卜素和圆酵母烯），并达到了如下最大浓度：182.0、43.9、23.0微克/克干细胞。(c) 1994约翰·威利父子公司。
