Bo Shaobo, Ni Xiaoxia, Guo Jintang, Liu Zhengyang, Wang Xiaoya, Sheng Yue, Zhang Genlin, Yang Jinfeng
Key Laboratory for Green Processing of Chemical Engineering of Xinjiang Bingtuan, School of Chemistry and Chemical Engineering, Shihezi University, Shihezi, China.
Front Nutr. 2022 Jun 17;9:918240. doi: 10.3389/fnut.2022.918240. eCollection 2022.
, as a member of the family , is of great value in the field of biotechnology. However, the evolutionary relationship of X-20 with , , and are not well understood, and its metabolic pathways such as carotenoid biosynthesis are not well resolved. Here, genome sequencing and comparative genome techniques were employed to improve the understanding of X-20. Phytoene desaturase (crtI) and 15-cis-phytoene synthase/lycopene beta-cyclase (crtYB), key enzymes in carotenoid pathway from X-20 were more efficiently expressed in INVSc1 than in CEN.PK2-1C. High yielding engineered strains were obtained by using synthetic biology technology constructing carotenoid pathway in and optimizing the precursor supply after fed-batch fermentation with palmitic acid supplementation. Genome sequencing analysis and metabolite identification has enhanced the understanding of evolutionary relationships and metabolic pathways in X-20, while heterologous construction of carotenoid pathway has facilitated its industrial application.
作为该家族的一员，在生物技术领域具有重要价值。然而，X - 20与[其他物种名称未给出]、[其他物种名称未给出]和[其他物种名称未给出]的进化关系尚未完全明晰，其类胡萝卜素生物合成等代谢途径也未得到很好的解析。在此，采用基因组测序和比较基因组技术来增进对X - 20的了解。来自X - 20的类胡萝卜素途径中的关键酶——八氢番茄红素去饱和酶（crtI）和15 - 顺式 - 八氢番茄红素合酶/番茄红素β - 环化酶（crtYB）在INVSc1中比在CEN.PK2 - 1C中表达更高效。通过使用合成生物学技术在[具体物种未给出]中构建类胡萝卜素途径，并在添加棕榈酸的补料分批发酵后优化前体供应，获得了高产工程菌株。基因组测序分析和代谢物鉴定增进了对X - 20进化关系和代谢途径的理解，而类胡萝卜素途径的异源构建促进了其工业应用。
