Innovative Institute for Plant Health, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, People's Republic of China.
College of Agriculture and Biology, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, People's Republic of China.
J Agric Food Chem. 2021 Sep 29;69(38):11523-11533. doi: 10.1021/acs.jafc.1c04028. Epub 2021 Sep 21.
Carotenoids are a group of tetraterpene pigments widely used in the food, pharmaceutical, and cosmetic industries. Torulene, torularhodin, and β-carotene, three principal carotenoids synthesized by ZHK, possess strong health-promoting properties such as antioxidant, provitamin A, and antitumor. Here, the effect of different salt conditions on carotenoids production of ZHK was investigated. The results showed that the total carotenoids were significantly enhanced in 0.5 M (3.91 mg/L) and 0.75 M (5.41 mg/L) NaCl treatments than that in 1.0 M (0.35 mg/L) and control (1.42 mg/L) after 120 h of cultivation. Of which, the increase in torulene and torularhodin production acts as the main contributor to the enhancement of total carotenoids. Transcriptome profiling revealed that salt stress efficiently promotes the gene expression of , which could explain the molecular mechanisms of the enhanced torulene and torularhodin production under salt stress. Further experiments indicated that torulene and torularhodin play an important role in quenching excrescent reactive oxygen species induced by salt stress. Together, the present study reports an effective strategy for simultaneously improving torulene and torularhodin production in ZHK.
类胡萝卜素是一组广泛应用于食品、制药和化妆品行业的四萜类色素。由 ZHK 合成的三种主要类胡萝卜素 torulene、torularhodin 和 β-胡萝卜素具有很强的促进健康的特性，如抗氧化、维生素 A 前体和抗肿瘤。在这里，研究了不同盐条件对 ZHK 类胡萝卜素生产的影响。结果表明，与 1.0 M（0.35 mg/L）和对照（1.42 mg/L）相比，在培养 120 h 后，0.5 M（3.91 mg/L）和 0.75 M（5.41 mg/L）NaCl 处理显著提高了总类胡萝卜素含量。其中，torulene 和 torularhodin 产量的增加是总类胡萝卜素增加的主要原因。转录组分析表明，盐胁迫有效地促进了基因的表达，这可以解释盐胁迫下 torulene 和 torularhodin 产量增加的分子机制。进一步的实验表明，torulene 和 torularhodin 在猝灭盐胁迫诱导的异常活性氧方面发挥着重要作用。总之，本研究报道了一种同时提高 ZHK 中 torulene 和 torularhodin 产量的有效策略。
