Zhang Ying, Chen Yao, Zhang Jin-Chao, Yang Meng-Su, Cao Hui, Xiao Pei-Gen
School of Chinese Materia Medica, Beijing University of Traditional Chinese Medicine, Beijing 100029, China.
Yao Xue Xue Bao. 2007 Jan;42(1):93-7.
To investigate the correlation between genotype and distribution of Pogostemon cablin by sequencing ITS1 and ITS2 genes, and provide molecular information for its germplasm evaluation, ITS1 and ITS2 genes of Pogostemon cablin from different localities were identified by PCR direct sequencing. The sequences of ITS1 and ITS2 genes were 424 bp and 380 bp in length, respectively. And nineteen base substitutions were observed in ITS1 gene, and five in ITS2 gene. The results showed a good correlation between genotype and distribution of Pogostemon cablin, and ITS gene sequencing could provide useful molecular information for germplasm evaluation of the plant species verification.
通过对广藿香ITS1和ITS2基因进行测序，研究其基因型与分布之间的相关性，并为其种质评价提供分子信息。采用PCR直接测序法对不同产地广藿香的ITS1和ITS2基因进行鉴定。ITS1和ITS2基因序列长度分别为424 bp和380 bp。在ITS1基因中观察到19个碱基替换，在ITS2基因中观察到5个碱基替换。结果表明广藿香的基因型与分布之间具有良好的相关性，ITS基因测序可为该植物物种鉴定的种质评价提供有用的分子信息。
