[溪黄草对D-氨基半乳糖诱导的大鼠急性肝损伤的保护作用]

[Protective effect of Isodon lophanthoides on acute hepatic injury induced by D-galactosamine in rats].

作者信息

Hou Shao-Zhen, Nagao Yukiko, Ye Mu-Rong, Zhu Chen-Chen, Li Geng, Lai Xiao-Ping

机构信息

College of Chinese Drug, Guangzhou University of TCM, Guangzhou 510405, China.

出版信息

Zhong Yao Cai. 2008 Feb;31(2):248-50.

PMID:18619271

Abstract

OBJECTIVE

To study the protective effects of Isodon lophanthoides (ILVG) aqueous extract on acute hepatic injury induced by D-galactosamine (D-Gal) in rats.

METHODS

60 rats were divided into control group, model group, and low, middle, high dosage group, Bifendate group randomly. In test groups, rats received either ILVG aqueous extract (15, 7.5, 3.75 g/kg) or Bifendate (45 mg/kg) by gastric perfusion daily for 7 consecutive days. On the sixth day, D-Gal (550 mg/kg) was given to rats by oral administration. The levels of ALT, AST, ALP, TBA, T-Bil, TP and ALB in serum were analyzed. The weight of body, liver, spleen and thymus of each rat were measured. The hepatic glycogen content was analyzed individually. Liver tissue pathology was observed.

RESULTS

ILVG coud decrease the ALT, AST, ALP, TBA and T-Bil in serum, increase TP, ALB and hepatic glycogen content and restrain the enlargement of liver and the shrinkage of thymus, reduce necrosis in pathological observation.

CONCLUSION

ILVG aqueous extract possesses the effect on protecting on acute hepatic injury induced by D-Gal in rats, its effect is related to multifarious mechanism.

摘要

目的

研究溪黄草水提取物对D-氨基半乳糖（D-Gal）诱导的大鼠急性肝损伤的保护作用。

方法

将60只大鼠随机分为对照组、模型组、低、中、高剂量组及联苯双酯组。试验组大鼠连续7天每日经胃灌注溪黄草水提取物（15、7.5、3.75 g/kg）或联苯双酯（45 mg/kg）。第6天，大鼠口服给予D-Gal（550 mg/kg）。分析血清中谷丙转氨酶（ALT）、谷草转氨酶（AST）、碱性磷酸酶（ALP）、总胆汁酸（TBA）、总胆红素（T-Bil）、总蛋白（TP）和白蛋白（ALB）的水平。测量每只大鼠的体重、肝脏、脾脏和胸腺重量。单独分析肝糖原含量。观察肝组织病理学变化。