Faith Minnie, Sukumaran Abitha, Pulimood Anna B, Jacob Molly
Department of Biochemistry, Christian Medical College, and Department of Gastrointestinal Sciences, The Wellcome Trust Research Laboratory, Christian Medical College Hospital, Vellore-632002, Tamil Nadu, India.
Clin Chim Acta. 2008 Oct;396(1-2):23-5. doi: 10.1016/j.cca.2008.06.016. Epub 2008 Jun 21.
Myeloperoxidase (MPO) and interleukin-6 (IL-6) are often used as markers of inflammation. The aim of this study was to ascertain whether MPO activity is as reliable as IL-6 as an indicator of inflammation.
Inflammation was induced in mice, using either turpentine or indomethacin. Duodenal tissue was removed from these animals at various time periods ranging from 6 h to 7 days later. Concentrations of IL-6 and MPO activity were estimated in the tissue. Histopathological examination was also carried out at some of the time periods to determine the presence of neutrophil infiltration in turpentine-treated mice.
Concentrations of IL-6 and MPO activity were significantly higher in tissue that had been treated with the agents used, at all the time periods studied, when compared with corresponding control tissue. Fold-increases in MPO activity were higher than fold-increases in IL-6. Concentrations of the 2 parameters showed significant positive correlation. Histopathological examination did not show significantly higher numbers of neutrophils infiltrating the tissue in response to turpentine, at the time periods studied.
Estimation of MPO activity is a reliable indicator of inflammation, being more sensitive than histopathological examination of tissue and as good as measurement of IL-6 concentrations.
髓过氧化物酶（MPO）和白细胞介素-6（IL-6）常被用作炎症标志物。本研究的目的是确定MPO活性作为炎症指标是否与IL-6一样可靠。
使用松节油或吲哚美辛在小鼠中诱导炎症。在6小时至7天后的不同时间段从这些动物身上取出十二指肠组织。估计组织中IL-6的浓度和MPO活性。在某些时间段还进行了组织病理学检查，以确定松节油处理的小鼠中是否存在中性粒细胞浸润。
在所有研究的时间段内，与相应的对照组织相比，用所用药物处理的组织中IL-6的浓度和MPO活性显著更高。MPO活性的倍数增加高于IL-6的倍数增加。这两个参数的浓度显示出显著的正相关。在所研究的时间段内，组织病理学检查未显示因松节油而浸润组织的中性粒细胞数量显著增加。
MPO活性的估计是炎症的可靠指标，比组织病理学检查更敏感，与IL-6浓度的测量效果相当。
