Ferreira Everton Allan, Queiroz Lucas Sales, Silva Facchini Gabriella de Faria, Guedes Maria Clara Machado Resende, Macedo Gilson Costa, de Sousa Orlando Vieira, Da Silva Filho Ademar A
Faculty of Pharmacy, Department of Pharmaceutical Sciences, Federal University of Juiz de Fora, Juiz de Fora, MG 36036-900, Brazil.
Department of Parasitology, Microbiology and Immunology, Federal University of Juiz de Fora, R. José Lourenço Kelmer s/n, Campus Universitário, 36036-900 Juiz de Fora, MG, Brazil.
Evid Based Complement Alternat Med. 2023 May 25;2023:9923941. doi: 10.1155/2023/9923941. eCollection 2023.
is popularly used to treat skin diseases. This work aimed to evaluate the topical anti-inflammatory properties of root extract (BdR) and its major compound baccharis oxide (BOx) on mice ear edema models. BdR was analyzed by GC-MS, and BOx was isolated by chromatographic fractionation. Topical anti-inflammatory activities were determined by using the croton oil, capsaicin, histamine, and phenol-induced mouse ear edema models. -acetyl--- glucosaminidase (NAG) and myeloperoxidase (MPO) activities, as well as NO dosage and histopathological analyses, were also evaluated. Phytochemical analysis of BdR showed BOx as one of the major constituents. BdR and BOx (both at 0.1, 0.5, and 1.0 mg/ear) significantly reduced croton oil, histamine, and phenol-induced ear edema, while only BOx was effective in reducing capsaicin-induced edema. MPO and NAG activities, as well as NO production, were significantly inhibited by BdR and BOx. Histopathological analysis confirmed the topical anti-inflammatory properties of BdR and BOx. Our findings showed that BdR and BOx demonstrated significant topical anti-inflammatory effects in mouse ear edema induced by different agents, suggesting their possible application on skin inflammatory diseases.
它被广泛用于治疗皮肤病。这项工作旨在评估根提取物（BdR）及其主要化合物氧化酒神菊（BOx）对小鼠耳部水肿模型的局部抗炎特性。通过气相色谱 - 质谱联用（GC-MS）分析BdR，并通过色谱分离法分离BOx。使用巴豆油、辣椒素、组胺和苯酚诱导的小鼠耳部水肿模型来测定局部抗炎活性。还评估了β - N - 乙酰氨基葡萄糖苷酶（NAG）和髓过氧化物酶（MPO）的活性，以及一氧化氮（NO）的含量和组织病理学分析。BdR的植物化学分析表明BOx是主要成分之一。BdR和BOx（均为0.1、0.5和1.0毫克/耳）显著减轻了巴豆油、组胺和苯酚诱导的耳部水肿，而只有BOx能有效减轻辣椒素诱导的水肿。BdR和BOx显著抑制了MPO和NAG的活性以及NO的产生。组织病理学分析证实了BdR和BOx的局部抗炎特性。我们的研究结果表明，BdR和BOx在不同试剂诱导的小鼠耳部水肿中表现出显著的局部抗炎作用，表明它们可能用于皮肤炎症性疾病。
