Dougherty W J, Dougherty M M, Harris S G
Department of Anatomy and Cell Biology.,Medical University of South Carolina, Charleston, South Carolina 29425, USA.
Tissue Cell. 1986;18(5):709-24. doi: 10.1016/0040-8166(86)90072-8.
Spermatophores obtained by electroejaculation from mature males of Macrobrachium rosenbergii were studied by light microscopy and by transmission and scanning electron microscopy. Upon electrical stimulation, single spermatophores were usually extruded simultaneously from each gonopore and frequently became firmly adherent to one another forming a compound spermatophore. Individual spermatophores were pod-shaped. 0.5-1 cm long, 2-3 microm in diameter and consisted of a lateral sperm mass, a medial mucus mass, and a noncellular capsule. The capsule was a single-layered PAS-reactive structure composed of fine, intertwining fibrils and almost completely surrounded the sperm and mucus masses. On its outer surface were large. AB-reactive globules of flocculent material partially surrounded by finger-like extensions of a canaliculated reticulum composed of fine, intertwining fibrils. The canaliculated reticulum may permit fluid imbibition by the spermatophore. Forming a central band within the capsule were many small membrane-less AB/PAS-reactive granules. The lateral sperm masses consisted of unistellate spermatozoa embedded in an AB/PAS-reaetive matrix composed of globules of flocculent. AB-reactive material and a highly plastic canaliculated and thread-filled reticulum. The reticulum itself consisted of fine, intertwining fibrils. The threads within the interstices of the reticulum were long, curving structures, 30-36 nm in diameter. The function(s) of the canaliculated and thread-filled reticulum within the sperm mass is not known at this time. The medial mucus mass was similar in fine structure and histochemical reactivity to the sperm mass, except that spermatozoa were lacking. It is apparent that the spermatophore of M. rosenbergii consists of relatively few complexly arranged structural and macromolecular components designed to transport and protect the spermatozoa.
通过电刺激从罗氏沼虾成熟雄性个体获得的精荚，利用光学显微镜、透射电子显微镜和扫描电子显微镜进行了研究。在电刺激下，单个精荚通常会同时从每个生殖孔挤出，并常常彼此牢固粘连形成复合精荚。单个精荚呈豆荚状，长0.5 - 1厘米，直径2 - 3微米，由外侧的精子团、中间的黏液团和无细胞的荚膜组成。荚膜是一层PAS反应性结构，由纤细、交织的纤维组成，几乎完全包围精子团和黏液团。在其外表面有大的、AB反应性的絮状物质小球，部分被由纤细、交织纤维组成的有小孔的网状结构的指状延伸部分包围。有小孔的网状结构可能允许精荚吸收液体。在荚膜内形成一条中央带的是许多小的无膜AB/PAS反应性颗粒。外侧的精子团由单星状精子嵌入由絮状小球、AB反应性物质和高度可塑性的有小孔且充满细丝的网状结构组成的AB/PAS反应性基质中。网状结构本身由纤细、交织的纤维组成。网状结构间隙内的细丝是长的、弯曲的结构，直径为30 - 36纳米。此时精子团内有小孔且充满细丝的网状结构的功能尚不清楚。中间的黏液团在精细结构和组织化学反应性方面与精子团相似，只是没有精子。很明显，罗氏沼虾的精荚由相对较少的、结构和大分子成分排列复杂的部分组成，旨在运输和保护精子。
