Martin Gary G, Herzig Chris, Narimatsu Glen
Department of Biology, Occidental College, Los Angeles, California 90041.
J Morphol. 1987 Jun;192(3):237-246. doi: 10.1002/jmor.1051920306.
Freshly extruded and hardened spermatophores of the spiny lobster, Panulirus interruptus, were compared using light and electron microscopy (EM). The spermatophore is composed of a sperm tube embedded in an acellular matrix. The sperm tube consists of tightly packed spherical cavities in an acellular material within which the sperm lie. The extruded spermatophore is white, soft, and sticky on all surfaces. The highly coiled sperm tube can be seen near the surface of the foot of the spermatophore, which is the side that will attach to the exoskeleton of the female. The opposite surface, the cap, will harden and darken after exposure to seawater. In the soft spermatophore, the matrix surrounding the sperm tube and extending from foot to cap is composed of small (2-μm) granules embedded in a loose weave of filaments. In the hardened spermatophore, the matrix is composed of small (4-μm) empty spheres. At the cap region the matrix darkens, and at the foot the granules dissolve to form a thick layer characterized by vertical striations. The structure of this spermatophore is compared to those spermatophores of other decapods that have been described at the EM level. The chemical composition and possible function(s) of the various components are discussed.
利用光学显微镜和电子显微镜（EM）对刚挤出并硬化的刺龙虾（Panulirus interruptus）精荚进行了比较。精荚由嵌入无细胞基质中的精子管组成。精子管由紧密排列在无细胞物质中的球形腔组成，精子位于其中。挤出的精荚呈白色，质地柔软，表面均有粘性。在精荚足部表面附近可以看到高度盘绕的精子管，该表面是将附着在雌性外骨骼上的一侧。相对的表面，即帽部，在接触海水后会硬化并变黑。在柔软的精荚中，围绕精子管并从足部延伸到帽部的基质由嵌入细丝松散编织物中的小颗粒（2微米）组成。在硬化的精荚中，基质由小的（4微米）空球体组成。在帽部区域，基质变黑，在足部，颗粒溶解形成一层以垂直条纹为特征的厚层。将这种精荚的结构与其他十足目动物在电子显微镜水平上描述的精荚结构进行了比较。讨论了各种成分的化学组成和可能的功能。
