从大鼠脾脏中分离出一种新型脂质半抗原——细胞脂质素S。

Isolation of a novel lipid hapten, cytolipin S, from rat spleen.

作者信息

Rapport M M, Graf L, Hungund B, Kisic A, Huang Y

出版信息

Chem Phys Lipids. 1976 Oct;17(2-3 SPEC NO):233-43. doi: 10.1016/0009-3084(76)90068-2.

Abstract

Antisera against rat erythrocytes contain agglutinins directed against unknown lipid determinants. Complement-fixation shows more reactivity with lipid extracts of rat spleen than of other rat tissues. The isolation of the reactive lipid from rat spleen, cytolipin S, is described. Cytolipin S is a glycosphingolipid containing glucose, galactose, and galactosamine with molar ratios of 1:2:1. It migrates on TLC like asialo GM1 (more slowly than cytolipin R, a ceramide tetrasaccharide, or cytolipin F, a ceramide pentasaccharide). Asialo GM1 and cytolipin S, when properly combined with auxiliary lipids, react very similarly with anti-rat erythrocyte sera by complement-fixation. However, cytolipin S is much more effective than asialo GM1 in inhibiting the hemagglutination reactions. It is concluded that cytolipin S and asialo GM1 are ceramide tetrasaccharides having different chemical structures and that the structural differences probably will be found in the carbohydrate linkages other than that between the terminal and penultimate residues.

摘要

抗大鼠红细胞抗血清含有针对未知脂质决定簇的凝集素。补体结合试验表明，与大鼠脾脏的脂质提取物相比，与其他大鼠组织的脂质提取物反应性更强。本文描述了从大鼠脾脏中分离出反应性脂质——细胞脂质S的过程。细胞脂质S是一种糖鞘脂，含有葡萄糖、半乳糖和氨基半乳糖，摩尔比为1:2:1。它在薄层层析（TLC）上的迁移情况与脱唾液酸GM1相似（比神经酰胺四糖细胞脂质R或神经酰胺五糖细胞脂质F迁移得慢）。当脱唾液酸GM1和细胞脂质S与辅助脂质适当结合时，通过补体结合试验，它们与抗大鼠红细胞血清的反应非常相似。然而，在抑制血凝反应方面，细胞脂质S比脱唾液酸GM1有效得多。得出的结论是，细胞脂质S和脱唾液酸GM1是具有不同化学结构的神经酰胺四糖，并且结构差异可能存在于除末端和倒数第二个残基之间以外的碳水化合物连接中。