Inoue K, Graf L, Rapport M M
J Lipid Res. 1972 Jan;13(1):119-27.
Rabbit antisera to rat lymphosarcoma contain antibodies that are cytolytic for rat erythrocytes in the presence of complement. The reaction can be inhibited completely by pure cytolipin R showing that (a) immune hemolysis can be mediated through lipid determinants in the membrane, and (b) that cytolipin R determinants are present in the intact erythrocyte membrane and exposed on the surface. Optimal conditions for measurement of cytolysis in this system based on release of (51)Cr are described. Degrees of specificity of a number of different antilymphosarcoma sera are shown, based on inhibition of cytolysis by cytolipin R, cytolipin K, cytolipin H, cytolipin F (F-hapten), glucocerebroside, galactocerebroside, ceramide trisaccharide (cer-glu-gal-gal), and a mixed brain ganglioside preparation. The data suggest that cytolytic antibodies and agglutinating antibodies in these antisera are distinctive despite their common specificity for cytolipin R. Lymphosarcoma cells are more effective than erythrocytes in absorbing cytolytic antibodies.
抗大鼠淋巴肉瘤的兔抗血清含有在补体存在下对大鼠红细胞具有细胞溶解作用的抗体。该反应可被纯细胞脂质R完全抑制，这表明：（a）免疫溶血可通过膜中的脂质决定簇介导；（b）细胞脂质R决定簇存在于完整的红细胞膜中并暴露于表面。描述了基于（51）Cr释放来测量该系统中细胞溶解的最佳条件。基于细胞脂质R、细胞脂质K、细胞脂质H、细胞脂质F（F-半抗原）、葡萄糖脑苷脂、半乳糖脑苷脂、神经酰胺三糖（神经酰胺-葡萄糖-半乳糖-半乳糖）和混合脑苷脂制剂对细胞溶解的抑制作用，展示了多种不同抗淋巴肉瘤血清的特异性程度。数据表明，尽管这些抗血清中的细胞溶解抗体和凝集抗体对细胞脂质R具有共同的特异性，但它们是有区别的。淋巴肉瘤细胞在吸收细胞溶解抗体方面比红细胞更有效。
