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器官和肿瘤脂质的免疫化学研究 XXI. 细胞脂质F - 绵羊红细胞系统的敏感性和特异性

Immunochemical studies of organ and tumor lipids XXI. Sensitivity and specificity of the cytolipin F - sheep erythrocyte system.

作者信息

Bathija A, Kisic A, Graf L, Rapport M M

出版信息

Connect Tissue Res. 1975;3(1):81-6. doi: 10.3109/03008207509152344.

DOI:10.3109/03008207509152344
PMID:51702
Abstract

The sensitivity and specificity of the sheep erythrocyte - anti-sheep erythrocyte system to inhibition by pure cytolipin F has been studied with 5 antisera, in order to compare it with the rat erythrocyte-anti-rat lymphosarcoma system and its inhibition by pure cytolipin R. The cytolipin F - sheep erythrocyte system is much more sensitive than the cytolipin R - rat erythrocyte system, inhibition of hemolysis of 6 x 10(6) sheep cells being produced by 10 ng of cytolipin F (combined with a four-fold quantity of lecithin) compared with inhibition of hemolysis of 10(6) rat cells by 50 to 100 ng of cytolipin R (also combined with lecithin). Differences in sensitivity are attributed to the larger number of available cytolipin F determinants on sheep erythrocytes compared with cytolipin R determinants on rat erythrocytes. Studies of the auxiliary lipid enhancement of cytolipin F activity by galactocerebroside, lactosyl ceramide (cytolipin H), and lecithin are also reported.

摘要

为了将其与大鼠红细胞 - 抗大鼠淋巴肉瘤系统及其被纯细胞脂质R抑制的情况进行比较,使用5种抗血清研究了绵羊红细胞 - 抗绵羊红细胞系统对纯细胞脂质F抑制的敏感性和特异性。细胞脂质F - 绵羊红细胞系统比细胞脂质R - 大鼠红细胞系统敏感得多,10 ng细胞脂质F(与四倍量的卵磷脂结合)可抑制6×10⁶个绵羊细胞的溶血,而50至100 ng细胞脂质R(也与卵磷脂结合)才能抑制10⁶个大鼠细胞的溶血。敏感性的差异归因于绵羊红细胞上可利用的细胞脂质F决定簇的数量比大鼠红细胞上的细胞脂质R决定簇更多。还报道了对半乳糖脑苷脂、乳糖基神经酰胺(细胞脂质H)和卵磷脂对细胞脂质F活性的辅助脂质增强作用的研究。

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Immunochemical studies of organ and tumor lipids XXI. Sensitivity and specificity of the cytolipin F - sheep erythrocyte system.器官和肿瘤脂质的免疫化学研究 XXI. 细胞脂质F - 绵羊红细胞系统的敏感性和特异性
Connect Tissue Res. 1975;3(1):81-6. doi: 10.3109/03008207509152344.
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