Santoro Nicola, Del Giudice Emanuele Miraglia, Grandone Anna, Marzuillo Pierluigi, Cozzolino Domenico, Di Salvo Giovanni, Pacileo Giuseppe, Calabrò Raffaele, Perrone Laura
Department of Pediatrics, Monaldi Hospital, Second University of Naples, Naples, Italy.
J Hypertens. 2008 Aug;26(8):1590-4. doi: 10.1097/HJH.0b013e32830413ed.
To verify whether peptide YY (PYY) and its Y2 receptor (Y2R) gene variants can be associated with obesity or hypertension or both in a cohort of obese children and adolescents.
Two hundred and twenty-nine obese children (105 girls, mean z-score BMI 5.1 +/- 2.4; mean age 10.5 +/- 2.9 years) and 250 age and sex-matched lean controls (130 women, mean z-score BMI 0.5 +/- 1.1; mean age 10.3 +/- 2.8) were enrolled in the study. Height, weight, BMI, waist circumference and 24-h systolic and diastolic blood pressure were measured. Night-time, day-time and 24-h systolic and diastolic blood pressures were evaluated by 24 h ambulatory blood pressure measurement, and appropriate standard deviation scores according to sex, age and height were calculated. Molecular screening of the PYY and Y2R genes was performed.
No new mutations were found. We observed three previously described polymorphisms: G767C on PYY and T585C and T936C on Y2R. An association study was carried out in obese patients. No associations were found between the PYY genotypes and the studied phenotypes. The Y2R gene variants, T585C and T936C, which are in almost complete linkage disequilibrium, were found to be associated with night-time, day-time and 24-h systolic and diastolic blood pressures. In particular, subject homozygotes for the T allele showed lower systolic and diastolic blood pressure values compared with the other genotypes. Moreover, obese children homozygous for the T585 allele showed a lower risk of developing hypertension than patients carrying the CC and CT genotypes (chi 6.9; df = 1, P = 0.03; odds ratio = 0.5, 95% confidence interval: 0.27-0.88).
Our results suggest that Y2R gene variants are involved in blood pressure regulation in obese children and adolescents.
在一组肥胖儿童和青少年中验证肽YY（PYY）及其Y2受体（Y2R）基因变异是否与肥胖或高血压或两者均相关。
本研究纳入了229名肥胖儿童（105名女孩，平均BMI z评分5.1±2.4；平均年龄10.5±2.9岁）和250名年龄及性别匹配的瘦对照者（130名女性，平均BMI z评分0.5±1.1；平均年龄10.3±2.8岁）。测量身高、体重、BMI、腰围以及24小时收缩压和舒张压。通过24小时动态血压测量评估夜间、日间及24小时收缩压和舒张压，并根据性别、年龄和身高计算相应的标准差分数。对PYY和Y2R基因进行分子筛查。
未发现新的突变。我们观察到三个先前描述的多态性：PYY上的G767C以及Y2R上的T585C和T936C。在肥胖患者中进行了关联研究。未发现PYY基因型与所研究表型之间存在关联。发现几乎完全连锁不平衡的Y2R基因变异T585C和T936C与夜间、日间及24小时收缩压和舒张压相关。特别是，T等位基因纯合子受试者的收缩压和舒张压值低于其他基因型。此外，T585等位基因纯合的肥胖儿童患高血压的风险低于携带CC和CT基因型的患者（χ² = 6.9；自由度 = 1，P = 0.03；优势比 = 0.5，95%置信区间：0.27 - 0.88）。
我们的结果表明，Y2R基因变异参与肥胖儿童和青少年的血压调节。
