Zsak L, Strother K O, Kisary J
Southeast Poultry Research Laboratory, USDA-ARS, Athens, GA 30605, USA.
Avian Pathol. 2008 Aug;37(4):435-41. doi: 10.1080/03079450802210648.
Poult enteritis mortality syndrome (PEMS) of turkeys and runting-stunting syndrome (RSS) of chickens are significant viral enteric diseases of poultry. Although a number of different viruses, including avian reoviruses, rotaviruses, astroviruses and coronaviruses, have been isolated from the intestinal contents of birds in affected poultry flocks, their role in PEMS and RSS is not yet understood. Here, we report the application of a molecular screening method to detection of novel viruses in intestinal samples of chickens and turkeys exhibiting characteristic signs of enteric disease. The technique is based on random amplification of particle-associated nucleic acids in clinical samples. Using this method we successfully identified parvovirus DNA sequences in intestinal homogenates of affected birds. This is the first time partial genomic sequences of autonomously replicating chicken and turkey parvoviruses have been described. Sequence analysis of the left end of the genome, including the complete non-structural gene, demonstrated that the chicken and turkey parvoviruses were closely related to each other and were representative of a novel member of the Parvovirus family. These parvoviruses may play a significant role in the aetiology of PEMS and RSS.
火鸡的禽肠炎死亡综合征（PEMS）和鸡的生长迟缓综合征（RSS）是家禽重要的病毒性肠道疾病。尽管从受影响家禽群的鸟类肠道内容物中分离出了多种不同的病毒，包括禽呼肠孤病毒、轮状病毒、星状病毒和冠状病毒，但它们在PEMS和RSS中的作用尚不清楚。在此，我们报告了一种分子筛选方法在检测表现出肠道疾病特征症状的鸡和火鸡肠道样本中的新型病毒方面的应用。该技术基于临床样本中与颗粒相关核酸的随机扩增。使用这种方法，我们在受影响鸟类的肠道匀浆中成功鉴定出细小病毒DNA序列。这是首次描述自主复制的鸡和火鸡细小病毒的部分基因组序列。对基因组左端的序列分析，包括完整的非结构基因，表明鸡和火鸡细小病毒彼此密切相关，是细小病毒科一个新成员的代表。这些细小病毒可能在PEMS和RSS的病因学中起重要作用。
