Szasz Bernadett K, Lenkey Nora, Barth Albert M, Mike Arpad, Somogyvari Zsolt, Farkas Orsolya, Lendvai Balazs
Department of Pharmacology, Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest, Hungary.
Planta Med. 2008 Aug;74(10):1235-9. doi: 10.1055/s-2008-1081292. Epub 2008 Jul 11.
In this study, an attempt was made to integrate the effects of GINKGO BILOBA extract (GBE) in different experimental systems (IN VITRO cochlea, brain slice preparations and cortical cell culture) to elucidate whether these processes converge to promote neuroprotection or interfere with normal neural function. GBE increased the release of dopamine in the cochlea. NMDA-evoked currents were dose-dependently inhibited by rapid GBE application in cultured cortical cells. GBE moderately inhibited Na+ channels at depolarised holding potential in cortical cells. These inhibitory effects by GBE may sufficiently contribute to the prevention of excitotoxic damage in neurons. However, these channels also interact with memory formation at the cellular level. The lack of effect by GBE on dendritic spike initiation in neocortical layer 5 pyramidal neurons indicates that the integrative functions may remain intact during the inhibitory actions of GBE.
在本研究中，我们尝试整合银杏叶提取物（GBE）在不同实验系统（体外耳蜗、脑片制备和皮质细胞培养）中的作用，以阐明这些过程是否共同促进神经保护或干扰正常神经功能。GBE增加了耳蜗中多巴胺的释放。在培养的皮质细胞中，快速应用GBE可剂量依赖性地抑制NMDA诱发的电流。GBE在皮质细胞去极化钳制电位下适度抑制钠通道。GBE的这些抑制作用可能足以预防神经元的兴奋性毒性损伤。然而，这些通道在细胞水平上也与记忆形成相互作用。GBE对新皮质第5层锥体神经元树突棘起始无影响，这表明在GBE的抑制作用期间，整合功能可能保持完整。
