Wang Congpin, Han Zhao
Department of Pharmacy, Eye and ENT Hospital of Fudan University, 83 Fenyang Road, Shanghai, 200031, China.
Cell Mol Neurobiol. 2015 Aug;35(6):861-9. doi: 10.1007/s10571-015-0180-z. Epub 2015 Mar 31.
Ginkgo biloba extract (GBE) has been widely used for treatment of neural damage and disorders. Neural stem cells (NSCs) hold promise as a treatment of hearing loss caused by neural damage. However, the biological functions of GBE in modulating NSC behaviors in the cochlea are still largely elusive. In this study, we sought to explore the effects of GBE on the differentiation and performance of NSCs from mouse cochlea. Our data showed that GBE treatment promotes cell survival and NSC proliferation. In addition, GBE treatment also increases NSC differentiation to neurons and enhances the performance of mature neural networks evident by the increased frequency of calcium oscillation. Moreover, neurite outgrowth is also dramatically increased upon GBE treatment. Overall, our study demonstrates the positive regulatory role of GBE in NSC proliferation and differentiation into functional neurons in vitro, supporting the potential therapeutic use of GBE in hearing loss recovery.
银杏叶提取物（GBE）已被广泛用于治疗神经损伤和疾病。神经干细胞（NSCs）有望成为治疗神经损伤导致的听力损失的方法。然而，GBE在调节耳蜗中NSC行为方面的生物学功能仍不清楚。在本研究中，我们试图探究GBE对小鼠耳蜗NSC分化和性能的影响。我们的数据表明，GBE处理可促进细胞存活和NSC增殖。此外，GBE处理还可增加NSC向神经元的分化，并通过增加钙振荡频率来增强成熟神经网络的性能。而且，GBE处理后神经突生长也显著增加。总体而言，我们的研究证明了GBE在体外对NSC增殖和分化为功能性神经元的正向调节作用，支持了GBE在听力损失恢复中的潜在治疗用途。
