Dunkelman N S, Zimber M P, Lebaron R G, Pavelec R, Kwan M, Purchio A F
Advanced Tissue Scinces, 10933 North Torrey Pines Road, La Jolla, California 92037-1005.
Biotechnol Bioeng. 1995 May 20;46(4):299-305. doi: 10.1002/bit.260460402.
Rabbit articular chondrocytes were seeded onto three-dimensional polyglycolic acid (PGA) scaffolds and placed into a closed bioreactor system. After 4 weeks of growth, meshes were examined for cartilage formation. Gross examination revealed solid, glistening material that had the appearance of cartilaginous tissue. Histologic examination revealed cell growth and deposition of extracellular matrix throughout the mesh with a less dense central core. Alcian blue and Safranin 0 staining showed deposition of glycosaminoglycans (GAGs). Immunostaining showed positive reactivity for type II collagen and chondroitin sulfate and no reactivity for type I collagen. Biochemical analysis showed collagen and GAG values to be 15% and 25% dry weight, respectively. Our results indicate that this type of system compares well with those previously described and should be useful for producing cartilage for evaluation in a clinical setting. (c) 1995 John Wiley & Sons, Inc.
将兔关节软骨细胞接种到三维聚乙醇酸（PGA）支架上，并置于封闭的生物反应器系统中。生长4周后，检查网格以观察软骨形成情况。大体检查发现有坚实、有光泽的物质，其外观类似软骨组织。组织学检查显示整个网格中有细胞生长和细胞外基质沉积，中心核心密度较低。阿尔辛蓝和番红O染色显示有糖胺聚糖（GAGs）沉积。免疫染色显示对II型胶原蛋白和硫酸软骨素呈阳性反应，对I型胶原蛋白无反应。生化分析表明，胶原蛋白和GAG的值分别为干重的15%和25%。我们的结果表明，这种类型的系统与先前描述的系统相比效果良好，应该有助于生产软骨用于临床评估。（c）1995约翰威立父子公司。
