Richardson F L
The Jackson Laboratory, Bar Harbor, Maine 04609, USA.
J Natl Cancer Inst. 1967 Aug;39(2):347-54.
The rate of development of mammary tumors in (C3H X RIII)F1 hybrid mice ovariectomized at 2 months of age was similar to that of mice ovariectomized at 3 months. The increased rate of tumor development in many females ovariectomized at 4 or 5 months over that of females ovariectomized at 2 or 3 months indicates that the ovaries before their removal affected the subsequent development of mammary tumors. In the controls, the mean tumor age was much lower and the tumor incidence higher than in the ovariectomized females. Nodular hyperplasia of the adrenals was present in all tumor-bearing mice spayed at 2 or 3 months of age and in many of those spayed at 4 or 5 months. These findings, with the use of F1 hybrids of C3H female X RIII hermaphrodite, agree with the postulate that hyperplastic adrenals of spayed mice of the C3H strain secrete estrogenic substances capable of inducing mammary tumors.
2月龄去卵巢的（C3H×RIII）F1杂种小鼠乳腺肿瘤的发生率与3月龄去卵巢的小鼠相似。4或5月龄去卵巢的许多雌性小鼠肿瘤发生率高于2或3月龄去卵巢的雌性小鼠，这表明切除卵巢前卵巢影响了乳腺肿瘤的后续发生。在对照组中，平均肿瘤年龄比去卵巢雌性小鼠低得多，肿瘤发生率更高。2或3月龄切除卵巢的所有荷瘤小鼠以及4或5月龄切除卵巢的许多小鼠中均存在肾上腺结节性增生。使用C3H雌性×RIII雌雄同体的F1杂种的这些发现，支持了C3H品系去卵巢小鼠增生的肾上腺分泌能够诱导乳腺肿瘤的雌激素物质这一假设。
