Ciulli Sara, Galletti Elena, Battilani Mara, Scagliarini Alessandra, Gentile Arcangelo, Morganti Luigi, Prosperi Santino
Department of Veterinary Public Health and Animal Pathology, Alma Mater Studiorum, University of Bologna, Ozzano Emilia, Italy.
New Microbiol. 2008 Apr;31(2):263-71.
Bovine Viral Diarrhoea Virus (BVDV) is responsible worldwide for severe economic losses on cattle farms. BVDV is an RNA virus with a high genome variability having practical consequences on epidemiology, diagnosis and disease control. Genetic monitoring was suggested as the first step in BVDV control. Thirty-seven Bovine Viral Diarrhoea Viruses were identified in persistently infected cattle, mucosal disease-affected animals and in bulk milk, and were characterised genetically. The 5'UTR region was amplified and sequenced, and a phylogenetic analysis was carried out comparing all the Italian sequences of BVDV available from the Genbank database. An unusual number of persistent infected animals was evidenced on more than one farm. Phylogenetic analysis attributed all our viruses to BVDV type I and distinguished four different subgroups inside this genotype. Analysis of old and new viruses revealed the circulation of viruses classified in subgroups BVDV Ia and Ij never reported in Italy.
牛病毒性腹泻病毒（BVDV）在全球范围内给养牛场造成了严重的经济损失。BVDV是一种RNA病毒，基因组变异性高，这对流行病学、诊断和疾病控制产生了实际影响。基因监测被建议作为控制BVDV的第一步。在持续感染的牛、患有黏膜病的动物和原料奶中鉴定出37种牛病毒性腹泻病毒，并对其进行了基因特征分析。扩增并测序了5'UTR区域，并进行了系统发育分析，比较了从Genbank数据库中获得的所有意大利BVDV序列。在不止一个农场发现了异常数量的持续感染动物。系统发育分析将我们所有的病毒归为BVDV I型，并在该基因型内区分出四个不同的亚组。对新旧病毒的分析揭示了在意大利从未报告过的BVDV Ia和Ij亚组中分类的病毒的传播情况。
