Barros Sílvia C, Ramos Fernanda, Paupério Sónia, Thompson Gertrude, Fevereiro Miguel
Laboratório Nacional de Investigação Veterinária, Dpt. Virologia, Lisboa, Portugal.
Virus Res. 2006 Jun;118(1-2):192-5. doi: 10.1016/j.virusres.2005.12.009. Epub 2006 Jan 26.
Bovine viral diarrhoea virus (BVDV) infections are an important cause of morbidity and mortality worldwide in dairy and beef cattle. To date, little is know about BVDV genotypes circulating in Portugal. For this purpose, a fragment within the 5'-untranslated region (5'-UTR) from 34 Portuguese field strains of BVDV was amplified by RT-PCR, cloned and sequenced. A maximum-likelihood phylogenetic analysis revealed that most of viruses, originated from cattle from different regions of the country, belong to BVDV type 1 (BVDV-1), genotypes 1b (n = 19), 1a (n = 6), 1d (n = 3) and 1e (n = 3); whereas three viruses clustered in BVDV type 2 (BVDV-2). The results from this study demonstrate that BVDV-lb is the most prevalent genotype and also shows the presence of BVDV-2 in Portugal.
牛病毒性腹泻病毒（BVDV）感染是全球范围内奶牛和肉牛发病和死亡的重要原因。迄今为止，对于葡萄牙境内传播的BVDV基因型知之甚少。为此，通过逆转录聚合酶链反应（RT-PCR）扩增、克隆并测序了来自34株葡萄牙BVDV田间毒株的5'-非翻译区（5'-UTR）内的一个片段。最大似然系统发育分析显示，大多数源自该国不同地区牛的病毒属于1型牛病毒性腹泻病毒（BVDV-1），基因型为1b（n = 19）、1a（n = 6）、1d（n = 3）和1e（n = 3）；而三种病毒聚集在2型牛病毒性腹泻病毒（BVDV-2）中。本研究结果表明，BVDV-1b是最普遍的基因型，同时也表明葡萄牙存在BVDV-2。
