Antoni Gunnar, Långström Bengt
GE Healthcare Uppsala Imanet AB, P.O. Box 967, 751 09, Uppsala, Sweden.
Handb Exp Pharmacol. 2008(185 Pt 1):177-201. doi: 10.1007/978-3-540-72718-7_9.
We describe the use of molecules labeled with short-lived emitting radionuclides for molecular imaging in combination with the positron emission tomography technique. How to use molecular probes to visualize and quantitatively determine rates of specific biochemical events such as synaptic transmission, enzymatic processes and binding to specific receptor proteins is highlighted. The sensitivity of the PET technique and the ability to measure and validate relationships between molecular events and biological functions is a key factor for the successful application of PET in biomedical research. In specific applications, the opportunity of using molecules labeled in specific positions may be critical. Molecular imaging using PET is also gaining increasing interest as a tool in drug development, especially when applied to early proof of concept studies in man. In this chapter, the concept of molecular imaging is exemplified and the use of position-specific labeling of tracer molecules as a tool to gain understanding of complex biological processes will be discussed.
我们描述了使用标记有短寿命发射放射性核素的分子，结合正电子发射断层扫描技术进行分子成像。重点介绍了如何使用分子探针来可视化和定量测定特定生化事件的速率，如突触传递、酶促过程以及与特定受体蛋白的结合。PET技术的灵敏度以及测量和验证分子事件与生物学功能之间关系的能力，是PET在生物医学研究中成功应用的关键因素。在特定应用中，使用特定位置标记分子的机会可能至关重要。使用PET进行分子成像作为药物开发工具也越来越受到关注，尤其是应用于人体早期概念验证研究时。在本章中，将举例说明分子成像的概念，并讨论使用示踪分子的位置特异性标记作为理解复杂生物过程的工具。
