Devchand M, Skipper N, Anton D L, DiCosimo R, Gavagan J E
Allelix Biopharmaceuticals Inc., 6850 Goreway Drive, Mississauga, Ontario, Canada L4V 1P1.
Biotechnol Bioeng. 1996 May 5;50(3):341-6. doi: 10.1002/(SICI)1097-0290(19960505)50:3<341::AID-BIT13>3.0.CO;2-Q.
The biocatalytic production of glyoxylic acid from glycolic acid requires two enzymes: glycolate oxidase, which catalyzes the oxidation of glycolic acid by oxygen to produce glyoxylic acid and hydrogen peroxide, and catalase, which decomposes the byproduct hydrogen peroxide. As an alternative to isolation from the leaf peroxisomes of spinach, glycolate oxidase has now been cloned and expressed in transformants of Aspergillus nidulans T580 at levels ranging from 1.7 to 36 IU/g dry wt. cells. The glycolate oxidase of transformant strain T17 comprises ca. 1.9% of total cell protein and is expressed at near 100% activity.
乙醇酸氧化酶，它催化乙醇酸被氧气氧化生成乙醛酸和过氧化氢；以及过氧化氢酶，它分解副产物过氧化氢。作为从菠菜叶片过氧化物酶体中分离的替代方法，乙醇酸氧化酶现已在构巢曲霉T580的转化体中克隆并表达，表达水平为1.7至36 IU/g干细胞重量。转化体菌株T17的乙醇酸氧化酶约占细胞总蛋白的1.9%，且以接近100%的活性表达。
