葡萄糖氧化酶——概述
Glucose oxidase--an overview.
作者信息
Bankar Sandip B, Bule Mahesh V, Singhal Rekha S, Ananthanarayan Laxmi
机构信息
Food Engineering and Technology Department, Institute of Chemical Technology, University of Mumbai, Matunga, Mumbai 400019, India.
出版信息
Biotechnol Adv. 2009 Jul-Aug;27(4):489-501. doi: 10.1016/j.biotechadv.2009.04.003. Epub 2009 Apr 15.
Glucose oxidase (beta-D-glucose:oxygen 1-oxidoreductase; EC 1.1.2.3.4) catalyzes the oxidation of beta-D-glucose to gluconic acid, by utilizing molecular oxygen as an electron acceptor with simultaneous production of hydrogen peroxide. Microbial glucose oxidase is currently receiving much attention due to its wide applications in chemical, pharmaceutical, food, beverage, clinical chemistry, biotechnology and other industries. Novel applications of glucose oxidase in biosensors have increased the demand in recent years. Present review discusses the production, recovery, characterization, immobilization and applications of glucose oxidase. Production of glucose oxidase by fermentation is detailed, along with recombinant methods. Various purification techniques for higher recovery of glucose oxidase are described here. Issues of enzyme kinetics, stability studies and characterization are addressed. Immobilized preparations of glucose oxidase are also discussed. Applications of glucose oxidase in various industries and as analytical enzymes are having an increasing impact on bioprocessing.
葡萄糖氧化酶（β-D-葡萄糖：氧1-氧化还原酶；EC 1.1.2.3.4）通过利用分子氧作为电子受体，同时产生过氧化氢，催化β-D-葡萄糖氧化为葡萄糖酸。微生物葡萄糖氧化酶由于其在化学、制药、食品、饮料、临床化学、生物技术和其他行业的广泛应用，目前受到了广泛关注。近年来，葡萄糖氧化酶在生物传感器中的新应用增加了对其的需求。本综述讨论了葡萄糖氧化酶的生产、回收、表征、固定化及应用。详细介绍了通过发酵生产葡萄糖氧化酶以及重组方法。这里描述了各种用于更高回收率的葡萄糖氧化酶的纯化技术。还讨论了酶动力学、稳定性研究和表征等问题。也讨论了葡萄糖氧化酶的固定化制剂。葡萄糖氧化酶在各个行业以及作为分析酶的应用对生物加工的影响越来越大。
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