Rampino N J, Chrambach A
Section on Macromolecular Analysis, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892.
Anal Biochem. 1991 May 1;194(2):278-83. doi: 10.1016/0003-2697(91)90230-q.
Apparatus for gel electrophoresis with continuous scanning by video epifluorescence microscopy was constructed and applied to measurements on individual DNA molecules in the size range of several hundred kilobase pairs. The gel electrophoresis apparatus comprises a horizontal glass cell and several commercially available components--an epifluorescence microscope, an image intensifier, two video cameras, a monitor, videotape recorder and voltmeter. Data in the form of videotapes can be subsequently analyzed on a computer graphics work station.
构建了用于通过视频落射荧光显微镜进行连续扫描的凝胶电泳装置，并将其应用于对数百千碱基对大小范围内的单个DNA分子的测量。该凝胶电泳装置包括一个水平玻璃槽和几个市售组件——一台落射荧光显微镜、一个图像增强器、两台摄像机、一台监视器、一台录像机和一台电压表。录像带形式的数据随后可在计算机图形工作站上进行分析。
