Zhang Yue-Kang, Liu Wen-Ke, Ma Lu
Department of Neurosurgery, West China Hospital, Sichuan University, Chengdu 610041, China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2008 Mar;39(2):211-3, 242.
To observe the variation of endothelin (ET) and nitric oxide (NO) in plasma and cerebrospinal fluid (CSF) in rabbits, and evaluate the effects of tetramethylpyrazine (TMP) on the prevention and cure of cerebral vasospasm (CVS) after subarachnoid hemorrhage (SAH).
24 New Zealand rabbits were randomly assigned into three groups: contrast group, experiment group and blank group. Every group contained 8 rabbits. SAH was established according to inject blood into the cisterna magna. The experiment group was administrated with TMP (20 mg/kg x d) transperitoneally. ET and NO of plasma and CSF were detected by radical immunoassay at 72 h and 168 h after SAH. Neurofunction were detected in every group at all the time scales.
(1) After SAH, the level of ET in CSF increased significantly in contrast group compared with that in experiment and blank groups (P<0.05). The value of ET at 168 h was higher than that at 72 h. The level of ET in plasma increased significantly in contrast group compared with blank and experiment groups (P<0.05), and no significant contrast could be found between blank and experiment group. (2) After SAH, the value of NO in CSF was lower in contrast group than in other groups (P<0.05), and the level of NO in CSF continued to decrease in all groups on some extent. As time went by, no significant contrast could be found in all groups. The value of NO in plasma was lower in contrast group than in other groups (P<0.05). There was no significant difference between experiment and blank groups. (3) The neuro-function score continued to be increased in contrast group, but decreased in experiment one. The neuro-function score was lower in experiment group than in contrast one at every time point (P<0.05).
After administration of TMP, the variation of ET has the continued decrease in plasma and CSF; the variation of NO shows the continued increase in plasma and CSF; neurological function gets possibly protected. TMP may prevent from and cure CVS after SAH.
观察兔血浆及脑脊液中内皮素（ET）和一氧化氮（NO）的变化，评价川芎嗪（TMP）对蛛网膜下腔出血（SAH）后脑血管痉挛（CVS）的防治作用。
将24只新西兰兔随机分为三组：对照组、实验组和空白组，每组8只。采用枕大池注血法制备SAH模型。实验组腹腔注射TMP（20mg/kg×d）。于SAH后72h和168h采用放射免疫法检测血浆及脑脊液中ET和NO含量，各时间点检测各组神经功能。
（1）SAH后，对照组脑脊液中ET水平较实验组和空白组显著升高（P<0.05），168h时ET值高于72h。对照组血浆中ET水平较空白组和实验组显著升高（P<0.05），空白组与实验组间无显著差异。（2）SAH后，对照组脑脊液中NO值低于其他组（P<0.05），各组脑脊液中NO水平均有不同程度下降，随时间推移，各组间无显著差异。对照组血浆中NO值低于其他组（P<0.05），实验组与空白组间无显著差异。（3）对照组神经功能评分持续升高，实验组则降低，各时间点实验组神经功能评分均低于对照组（P<0.05）。
应用TMP后，血浆及脑脊液中ET变化呈持续下降；NO变化呈持续升高；神经功能可能得到保护。TMP可能对SAH后CVS具有防治作用。
