Ma Lu, Liu Wen-Ke, Zhang Yue-Kang, Ju Yan
Department of Neurosurgery, West China Hospital, Sichuan University, Chengdu 610041, China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2008 Mar;39(2):207-10.
To observe the expression levels of BDNF and bFGF, the variation of superoxide dismutase (SOD) and malondialdehyde (MDA) in serum and injury neuron of rat, and to evaluate the effect of Tetramethylpyrazine (TMP) on neuro-protection finally.
120 SD rats were randomly assigned into three groups: pseudo-operation group, severe brain injury model group and TMP treatment group. Every group contained 40 rats. The severe brain injury (SBI) of rat was established according to Feeney' s method. The treatment group was administrated with TMP transperitoneally. Rats were killed at 7 h, 24 h, 72 h, 168 h respectively after SBI. BDNF, bFGF, Nissl body and neurofunction were detected in every group at all the time scales. MDA and SOD of serum were also detected by radical immunoassay.
After rat SBI treated with the administration of TMP, the expression of BDNF and bFGF increased significantly in the injured brain tissue (P<0.05), and the MDA level showed to be decreased but the SOD was increased in serum (P<0.05). The Nissl body and neuro-function were well protected.
TMP may protect the rat cerebral neurons from SBI.
