肥厚性瘢痕和瘢痕疙瘩中Fas蛋白表达的免疫组织化学分析
Immunohistochemical analysis of Fas protein expression in hypertrophic scar and keloid.
作者信息
Lu Feng, Gao Jian-hua, Li Xiao-jian
机构信息
Department of Plastic Surgery, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China.
出版信息
Di Yi Jun Yi Da Xue Xue Bao. 2003 Mar;23(3):228-9, 232.
OBJECTIVE
To find an efficient diagnostic method of hypertrophic scar and keloid.
METHODS
Immunohistochemistry was employed to detect the expression and distribution of Fas protein in keloids, hypertrophic scars and their surrounding normal skins. Image analysis was performed to quantitatively compare the differences of the protein expression in these tissues.
RESULTS
The expression level of Fas antigen was much higher in the keloids and their surrounding normal skins than in hypertrophic scars, and no significant difference in the expression level between the former 2 tissues was found. In hypertrophic scars, however, much lower Fas protein expression was detected in comparison with that in their surrounding normal skins(P < 0.01).
CONCLUSION
Immunohistochemical detection of Fas protein is a simple method that is well applicable in the differential diagnosis of pathological scars.
目的
寻找一种有效的肥厚性瘢痕和瘢痕疙瘩诊断方法。
方法
采用免疫组织化学法检测瘢痕疙瘩、肥厚性瘢痕及其周围正常皮肤中Fas蛋白的表达及分布。进行图像分析以定量比较这些组织中蛋白表达的差异。
结果
瘢痕疙瘩及其周围正常皮肤中Fas抗原的表达水平远高于肥厚性瘢痕，且前两者之间的表达水平无显著差异。然而，在肥厚性瘢痕中，与周围正常皮肤相比，Fas蛋白表达检测到低得多（P < 0.01）。
结论
Fas蛋白的免疫组织化学检测是一种简单的方法，适用于病理性瘢痕的鉴别诊断。
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