Abd-Aziz Suraini, Fernandez Christine Cheryl, Salleh Madihah Md, Illias Rosli Md, Hassan Mohd Ali
Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
Appl Biochem Biotechnol. 2008 Aug;150(2):193-204. doi: 10.1007/s12010-008-8140-4. Epub 2008 Feb 26.
Shrimps have been a popular raw material for the burgeoning marine and food industry contributing to increasing marine waste. Shrimp waste, which is rich in organic compounds is an abundant source of chitin, a natural polymer of N-acetyl-D-glucosamine (GluNac), a reducing sugar. For this respect, chitinase-producing fungi have been extensively studied as biocontrol agents. Locally isolated Trichoderma virens UKM1 was used in this study. The effect of agitation and aeration rates using colloidal chitin as control substrate in a 2-l stirred tank reactor gave the best agitation and aeration rates at 200 rpm and 0.33 vvm with 4.1 U/l per hour and 5.97 U/l per hour of maximum volumetric chitinase activity obtained, respectively. Microscopic observations showed shear sensitivity at higher agitation rate of the above system. The oxygen uptake rate during the highest chitinase productivity obtained using sun-dried ground shrimp waste of 1.74 mg of dissolved oxygen per gram of fungal biomass per hour at the kappaL a of 8.34 per hour.
虾类一直是新兴海洋和食品工业的热门原材料，这导致海洋废弃物不断增加。虾类废弃物富含有机化合物，是几丁质的丰富来源，几丁质是N-乙酰-D-葡萄糖胺（氨基葡萄糖）的天然聚合物，氨基葡萄糖是一种还原糖。鉴于此，产几丁质酶的真菌作为生物防治剂已得到广泛研究。本研究使用了本地分离的绿色木霉UKM1。在2升搅拌罐式反应器中，以胶体几丁质作为对照底物，研究搅拌和通气速率的影响，结果表明，在200转/分钟和0.33体积空气每分钟的条件下，搅拌和通气速率最佳，最大体积几丁质酶活性分别为每小时4.1酶单位/升和每小时5.97酶单位/升。显微镜观察显示，上述系统在较高搅拌速率下具有剪切敏感性。在使用晒干磨碎的虾类废弃物时，每克真菌生物量每小时的溶解氧摄取率为1.74毫克，此时几丁质酶生产率最高，传质系数为每小时8.34。
