Department of Biotechnology and Food Microbiology, Wrocław University of Environmental and Life Sciences, Wrocław, Poland.
Prep Biochem Biotechnol. 2012;42(3):279-91. doi: 10.1080/10826068.2012.656868.
The effects of agitation rates from 400 to 900 rpm and aeration rates ranging from 0.18 to 0.6 vvm on biomass and citric acid production on glycerol media by acetate-negative mutants of Yarrowia lipolytica, Wratislavia 1.31 and Wratislavia AWG7, in batch culture were studied. The agitation rates of 800 and 900 rpm (at a constant aeration rate of 0.36 vvm) and aeration rates within the range of 0.24-0.48 vvm (at a constant agitation rate of 800 rpm), which generated dissolved oxygen concentration (DO) higher than 40%, were found the best for citric acid biosynthesis from glycerol. An increase in agitation rate (higher than 800 rpm) and aeration rate (higher than 0.36 vvm) had no impact on DO and citric acid production. The highest citric acid concentration (92.8 g/L) and yield (0.63 g/g) were obtained with Wratislavia 1.31 strain at 0.24 vvm. The highest volumetric citric acid production rate (1.15 g/Lh) and specific citric acid production rate (0.071 g/gh) were reached at 0.48 vvm.
研究了在静置培养条件下，搅拌转速从 400 到 900rpm 以及通气速率从 0.18 到 0.6vvm 对乙酸盐阴性突变株脂肪假丝酵母、Wratislavia 1.31 和 Wratislavia AWG7 利用甘油生产生物量和柠檬酸的影响。发现转速为 800 和 900rpm（通气速率为 0.36vvm 时保持恒定）以及通气速率在 0.24-0.48vvm 范围内（搅拌转速为 800rpm 时保持恒定）有利于从甘油生产柠檬酸，因为这两种条件下产生的溶解氧浓度（DO）都高于 40%。转速（高于 800rpm）和通气速率（高于 0.36vvm）的增加对 DO 和柠檬酸的生产没有影响。在 0.24vvm 下，Wratislavia 1.31 菌株获得了最高的柠檬酸浓度（92.8g/L）和产率（0.63g/g）。在 0.48vvm 下，获得了最高的柠檬酸比生产速率（1.15g/Lh）和特定柠檬酸比生产速率（0.071g/gh）。
