Shandong Provincial Key Laboratory of Microbial Engineering, School of Food and Bioengineering, Qilu University of Technology, Jinan, 250353 China.
Indian J Microbiol. 2014 Dec;54(4):459-65. doi: 10.1007/s12088-014-0485-5. Epub 2014 Jul 8.
Glucosamine (GlcN) is a major and valuable component in the cell wall of fungi. In this study, the cell wall was treated via a two-stage alkali and acid process, and chitin and chitosan were fully deacetylated, partially depolymerized, and converted to GlcN oligosaccharides. Then, the oligosaccharides were analyzed by high performance liquid chromatography. The influences of Actinomucor elegans on GlcN production in a flask culture were investigated to achieve an optimum yield of GlcN. The experimental result showed that cultivation in condition of pH 6.0, 100 mL working volume (500 mL flask), 10 % (v/v) inoculum concentration, at 28 °C and 200 rpm for 6 days yielded highest dry cell weight (DCW) which was 23.43 g L(-1), with a GlcN concentration of 5.12 g L(-1). Methanol as stimulating factor was found to exert the best effect in concentration of 1.5 % (v/v). With addition of methanol into medium, the DCW increased from 23.69 to 32.42 g L(-1), leading to maximum GlcN concentration of 6.85 g L(-1) obtained. Here, the methanol addition may be useful for industrial production of GlcN, and may also be meaningful for the production of other fine chemicals by filamentous fungi.
氨基葡萄糖（GlcN）是真菌细胞壁的主要且有价值的成分。在本研究中，通过两步碱酸处理细胞壁，壳聚糖完全脱乙酰化，部分解聚，并转化为 GlcN 低聚糖。然后，通过高效液相色谱法对低聚糖进行分析。研究了食线虫毛霉对摇瓶培养中 GlcN 生产的影响，以获得 GlcN 的最佳产量。实验结果表明，在 pH 6.0、100 mL 工作体积（500 mL 摇瓶）、10%（v/v）接种浓度、28°C 和 200 rpm 下培养 6 天，可获得最高的干细胞重量（DCW），为 23.43 g L(-1)，GlcN 浓度为 5.12 g L(-1)。发现甲醇作为刺激因子在 1.5%（v/v）的浓度下效果最佳。向培养基中添加甲醇，DCW 从 23.69 增加到 32.42 g L(-1)，导致获得最大 GlcN 浓度 6.85 g L(-1)。在这里，甲醇的添加可能对 GlcN 的工业生产有用，并且对丝状真菌生产其他精细化学品也可能具有意义。
