Seki M, Ohzora C, Takeda M, Furusaki S
Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo, Tokyo 113, Japan.
Biotechnol Bioeng. 1997 Jan 20;53(2):214-9. doi: 10.1002/(SICI)1097-0290(19970120)53:2<214::AID-BIT12>3.0.CO;2-K.
The production characteristics for Taxol (paclitaxel) using free and immobilized cells of Taxus cuspidata were investigated in a perfusion culture bioreactor. Although the cell growth was inhibited by higher dilution rates, the specific production rate of Taxol was increased by perfusion compared with that using batch operation. Perfusion cultures using a nylon-mesh cell separator for free suspension cells showed similar production profiles to those obtained using immobilized cells. Continuous Taxol production was successfully obtained at an approximate specific production rate of 0.3 mg/g DCW (dry cell weight) per day for up to 40 days. (c) 1997 John Wiley & Sons, Inc.
在灌注培养生物反应器中，研究了利用东北红豆杉游离细胞和固定化细胞生产紫杉醇的特性。尽管较高的稀释率会抑制细胞生长，但与分批操作相比，灌注可提高紫杉醇的比生产速率。使用尼龙网细胞分离器对游离悬浮细胞进行的灌注培养显示出与固定化细胞相似的生产曲线。连续40天以约0.3 mg/g干细胞重量每天的比生产速率成功实现了紫杉醇的持续生产。(c) 1997约翰威立父子公司。
