Department of Chemistry (Analytical Chemistry), University of La Rioja, 26001 Logroño, La Rioja, Spain.
Biotechnol Bioeng. 1997 Mar 5;53(5):497-506. doi: 10.1002/(SICI)1097-0290(19970305)53:5<497::AID-BIT7>3.0.CO;2-C.
We have developed a methodology based on experimental design, to optimize a polyacrylamide gel as the support for enzyme immobilization, taking advantage of all the properties which this type of gel has. Monomer and crosslinking agent proportions are responsible for both the porous structure and pore size of the gel. A correct selection of those variables and suitable synthesis conditions leads to an increase in the activity retained by the gel. The path of steepest ascent method was used to obtain the relative maximum activity. The maximum retained activity was chosen with a central composite design in terms of the gel composition. The retained activity in the network, loss activity in the wash water, and loss activity due to steric impediment or blockage was modeled in terms of the variables responsible for the gel structure. (c) 1997 John Wiley & Sons, Inc. Biotechnol Bioeng 53: 497-506, 1997.
我们已经开发出一种基于实验设计的方法，以优化聚丙烯酰胺凝胶作为酶固定化的支持物，充分利用这种凝胶的所有特性。单体和交联剂的比例决定了凝胶的多孔结构和孔径。正确选择这些变量和合适的合成条件可以提高凝胶保留的活性。采用最速上升法获得相对最大活性。通过中心复合设计选择最大保留活性，以凝胶组成来表示。网络中保留的活性、在洗涤水中损失的活性以及由于空间位阻或堵塞而损失的活性都可以用负责凝胶结构的变量来建模。（c）1997 年 John Wiley & Sons，Inc.生物工艺学 53:497-506,1997.
