Department of Chemical Engineering, University of Rome La Sapienza, via Eudossiana 18, 00184 Rome, Italy.
Biotechnol Bioeng. 1997 Jul 20;55(2):261-6. doi: 10.1002/(SICI)1097-0290(19970720)55:2<261::AID-BIT3>3.0.CO;2-G.
Large phosphatydilcholine unilamellar vesicles appear to be suitable controlled and protective delivery systems of beta-galactosidase. Kinetic measurements carried out on intact loaded liposomes show that most of the enzyme is entrapped inside the liposomes and its activity is latent. Nevertheless, intact liposomes also show significant activity, which can be controlled by addition of detergent. At sublytic detergent concentrations, liposome enzymatic activity reaches values two or three times greater than those of intact liposomes. This increase seems to be due to membrane structure modification that also enhances the substrate permeability across the bilayer. (c) 1997 John Wiley & Sons, Inc. Biotechnol Bioeng 55: 261-266, 1997.
大的磷脂酰胆碱单层囊泡似乎是β-半乳糖苷酶的合适的可控保护递药系统。对负载完整的脂质体进行的动力学测量表明，大多数酶被包封在脂质体内部，其活性是潜伏的。然而，完整的脂质体也显示出显著的活性，这种活性可以通过添加去污剂来控制。在亚毒性的去污剂浓度下，脂质体的酶活性达到比完整脂质体高两倍或三倍的值。这种增加似乎是由于膜结构的修饰，这也增强了底物穿过双层的通透性。（c）1997 年 John Wiley & Sons，Inc. 生物技术与生物工程 55:261-266，1997 年。
