Murnleitner E, Kuba T, van Loosdrecht M C, Heijnen J J
Department of Biochemical Engineering, Delft University of Technology, Julianalaan 67, 2628 BC Delft, The Netherlands.
Biotechnol Bioeng. 1997 Jun 5;54(5):434-50. doi: 10.1002/(SICI)1097-0290(19970605)54:5<434::AID-BIT4>3.0.CO;2-F.
In this work, an integrated metabolic model for biological phosphorus removal is presented. Using a previously proposed mathematical model it was shown to be possible to describe the two known biological phosphorus removal processes, under aerobic and denitrifying conditions, with the same biochemical reactions, where only the difference in electron acceptor (oxygen and nitrate) is taken into account. Though, apart from the ATP/NADH ratio, the stoichiometry in those models is identical, different kinetic parameters were found. Therefore, a new kinetic structure is proposed that adequately describes phosphorus removal under denitrifying and aerobic conditions with the same kinetic equations and parameters. The ATP/NADH ratio (delta) is the only model parameter that is different for aerobic and denitrifying growth. With the new model, simulations of anaerobic/aerobic and anaerobic/denitrifying sequencing batch reactors (A(2) SBR and A/O SBR) were made for verification of the model. Not only short-term behavior, but also steady state, was simulated. The results showed very good agreement between model predictions and experimental results for a wide range of dynamic conditions and sludge retention times. Sensitivity analysis shows the influence of the model parameters and the feed substrate concentrations on both systems.
在这项工作中，提出了一种用于生物除磷的综合代谢模型。使用先前提出的数学模型表明，在有氧和反硝化条件下，利用相同的生化反应来描述两种已知的生物除磷过程是可行的，其中仅考虑电子受体（氧气和硝酸盐）的差异。然而，除了ATP/NADH比率外，这些模型中的化学计量是相同的，但发现了不同的动力学参数。因此，提出了一种新的动力学结构，该结构用相同的动力学方程和参数充分描述了反硝化和好氧条件下的除磷过程。ATP/NADH比率（δ）是好氧和反硝化生长中唯一不同的模型参数。利用新模型，对厌氧/好氧和厌氧/反硝化序批式反应器（A(2) SBR和A/O SBR）进行了模拟，以验证该模型。不仅模拟了短期行为，还模拟了稳态。结果表明，在广泛的动态条件和污泥停留时间下，模型预测与实验结果非常吻合。敏感性分析显示了模型参数和进料底物浓度对两个系统的影响。
