Devash Y, Hauschner A, Sela I, Chakraburtty K
Virus Laboratory, Hebrew University, Faculty of Agriculture, Rehovot, Israel.
Virology. 1981 May;111(1):103-12. doi: 10.1016/0042-6822(81)90657-7.
Crude preparations of the antiviral factor (AVF) exhibit also another activity measured by their ability to discharge histidinyl-TMV-RNA. The "discharging factor" (DF) is separable from AVF and the DF activity is released in AVF-treated leaves. AVF induces DF activity in leaves of Nicotiana glutinosa which carries the N-gene, as well as in the tobacco cultivar "Samsun" where the N-gene is absent. The DF activity is mediated by a transient low-molecular-weight compound, probably a polymerized ATP, which can be isolated from TMV-infected N. glutinosa leaves. A fraction capable of polymerizing ATP was indeed isolated from AVF-treated leaf extracts, and the polymerized product was able to revive inactive DF preparations. A nuclease activity specific to crude AVF preparations is also reported. The similarity of the AVF to the interferon systems is discussed.
抗病毒因子（AVF）的粗制品还表现出另一种活性，可通过其释放组氨酰 - TMV - RNA的能力来衡量。“释放因子”（DF）可与AVF分离，且DF活性在经AVF处理的叶片中释放。AVF在携带N基因的粘毛烟草叶片以及不含N基因的烟草品种“萨姆松”的叶片中均可诱导DF活性。DF活性由一种短暂存在的低分子量化合物介导，可能是聚合ATP，它可从感染TMV的粘毛烟草叶片中分离得到。实际上，从经AVF处理的叶片提取物中分离出了一种能够聚合ATP的组分，并且该聚合产物能够使无活性的DF制品恢复活性。还报道了粗制AVF制品具有的一种核酸酶活性。文中讨论了AVF与干扰素系统的相似性。
