Reichman M, Devash Y, Suhadolnik R J, Sela I
Virus Laboratory, The Hebrew University, Faculty of Agriculture, Rehovot 76100, Israel.
Virology. 1983 Jul 15;128(1):240-4. doi: 10.1016/0042-6822(83)90334-3.
Leaves of Nicotiana glutinosa and tobacco, as well as tobacco callus cultures, were treated with the plant antiviral factor AVF or with a purified subspecies of human leukocyte interferon. After incubation, a fraction containing short oligonucleotides was extracted directly from the plant tissue. In addition, a synthetase preparation was fractionated from treated tissues, which polymerized ATP in the presence of polyinosinic, polycytidylic acid (poly(I:C)). The various plant nucleotides were applied to tobacco mosaic virus (TMV)-infected leaf disks, the TMV content of which were determined by an enzyme-linked immunosorbent assay. The nucleotide fraction extracted directly from TMV-infected leaves exhibited a considerable antiviral activity, whereas similar fractions from AVF- or interferon-treated leaves did not, even though an antiviral state was induced in the tissue by these agents. However, when the synthetase fraction from TMV-infected, AVF-treated, or interferon-treated tissues was incubated with ATP and poly(I:C), the resultant, heat-stable, acid-soluble, polymerized ATP markedly inhibited TMV multiplication. It is concluded that the presence of double-stranded RNA is obligatory for the formation of the antiviral nucleotides. The analogy to interferon-induced resistance in animal tissues is discussed.
用植物抗病毒因子AVF或人白细胞干扰素的纯化亚种处理粘性烟草和烟草的叶片以及烟草愈伤组织培养物。孵育后，直接从植物组织中提取含有短寡核苷酸的部分。此外，从处理过的组织中分离出一种合成酶制剂，该制剂在聚肌苷酸、聚胞苷酸（聚（I:C））存在下使ATP聚合。将各种植物核苷酸应用于感染烟草花叶病毒（TMV）的叶盘，通过酶联免疫吸附测定法测定其TMV含量。直接从感染TMV的叶片中提取的核苷酸部分表现出相当大的抗病毒活性，而来自AVF或干扰素处理叶片的类似部分则没有，尽管这些试剂在组织中诱导了抗病毒状态。然而，当将来自感染TMV、AVF处理或干扰素处理组织的合成酶部分与ATP和聚（I:C）一起孵育时，所得的热稳定、酸溶性、聚合ATP显著抑制TMV增殖。得出结论，双链RNA的存在对于抗病毒核苷酸的形成是必不可少的。讨论了与动物组织中干扰素诱导抗性的类比。
