Rezaian M A, Jackson A O
Department of Biology, Shiraz University, Shiraz, Iran.
Virology. 1981 Oct 31;114(2):534-41. doi: 10.1016/0042-6822(81)90233-6.
The low-molecular-weight RNA associated with an isolate of tobacco ringspot virus [TRSV(F)] lacks polyadenylic acid [poly(A)] sequences. Complementary DNA (cDNA) specific to this RNA was made with an oligo(dT) primer after tailing the RNA with poly(A). However, a significant amount of cDNA was made only when the RNA was treated with alkaline phosphatase prior to tailing. Hybridization of RNA in solution and by a Northern blot technique showed that there was no detectable nucleotide sequence homology between TRSV(F) RNA and two of the low-molecular-weight RNAs associated with this virus. These associated RNAs are therefore, satellites of TRSV(F). The relationship of these satellites to a previously reported satellite of TRSV is discussed.
与烟草环斑病毒分离株[TRSV(F)]相关的低分子量RNA缺乏聚腺苷酸[poly(A)]序列。在用聚(A)对该RNA进行加尾后，使用寡聚(dT)引物制备了该RNA特异的互补DNA(cDNA)。然而，只有在加尾前用碱性磷酸酶处理RNA时，才能产生大量的cDNA。溶液中RNA的杂交以及Northern印迹技术表明，TRSV(F)RNA与该病毒相关的两种低分子量RNA之间没有可检测到的核苷酸序列同源性。因此，这些相关RNA是TRSV(F)的卫星RNA。讨论了这些卫星RNA与先前报道的TRSV卫星RNA的关系。
