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一种核苷酸序列重排区分了卫星烟草环斑病毒RNA的两个分离株。

A nucleotide sequence rearrangement distinguishes two isolates of satellite tobacco ringspot virus RNA.

作者信息

Buzayan J M, McNinch J S, Schneider I R, Bruening G

出版信息

Virology. 1987 Sep;160(1):95-9. doi: 10.1016/0042-6822(87)90049-3.

DOI:10.1016/0042-6822(87)90049-3
PMID:3629980
Abstract

Several strains of tobacco ringspot virus (TobRV) support the replication and encapsidation of satellite tobacco ringspot virus RNA (STobRV RNA). We have compared the nucleotide sequences of four STobRV RNAs, each initially associated with a different isolate of TobRV. A STobRV RNA from a geranium isolate of TobRV and STobRV RNA from the previously analyzed budblight isolate (J.M. Buzayan, W.L. Gerlach, G. Bruening, P. Keese, and A.R. Gould, 1986, Virology 151, 186-199) differed by a single nucleotide residue substitution. STobRV RNAs from TobRV isolates 62L and NC-87 have the same 360-residue nucleotide sequence. This sequence differs from that of the 359-nucleotide residue budblight STobRV RNA principally at locations 100 through 140. The differences between the two sequences in this region are consistent with a rearrangement of blocks of nucleotide residues. The two sequences can be folded with similar patterns of base pairing. All four STobRV RNAs share a sequence of eighty 5'-terminal and of twenty 3'-terminal residues, including the 5' hydroxyl group and 2':3'-cyclic phosphodiester group.

摘要

几种烟草环斑病毒(TobRV)毒株支持卫星烟草环斑病毒RNA(STobRV RNA)的复制和衣壳化。我们比较了四种STobRV RNA的核苷酸序列,每种最初都与不同的TobRV分离株相关。来自TobRV天竺葵分离株的STobRV RNA和先前分析的芽枯分离株的STobRV RNA(J.M.布扎扬、W.L.格拉赫、G.布鲁宁、P.基斯和A.R.古尔德,1986年,《病毒学》151卷,第186 - 199页)仅相差一个核苷酸残基替换。来自TobRV分离株62L和NC - 87的STobRV RNA具有相同的360个残基的核苷酸序列。该序列与359个核苷酸残基的芽枯STobRV RNA序列主要在100至140位不同。该区域两个序列之间的差异与核苷酸残基块的重排一致。这两个序列可以折叠成相似的碱基配对模式。所有四种STobRV RNA都共享一段由80个5'端和20个3'端残基组成的序列,包括5'羟基和2':3'-环磷酸二酯基团。

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A nucleotide sequence rearrangement distinguishes two isolates of satellite tobacco ringspot virus RNA.一种核苷酸序列重排区分了卫星烟草环斑病毒RNA的两个分离株。
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