Smit C H, Jaspars E M
Department of Biochemistry, State University of Leiden, P.O. Box 9505, 2300 RA Leiden, The Netherlands.
Virology. 1982 Feb;117(1):271-4. doi: 10.1016/0042-6822(82)90528-1.
A mutant (Tbts7) of alfalfa mosaic virus, the coat protein of which is unable to activate the viral genome (the RNA species 1, 2, and 3, which need some coat protein for infectivity) at 30 degrees , can be rescued at this temperature by adding to the inoculum wild-type RNA 3 (the genome part that contains the coat protein cistron), but not adding wild-type RNA 4 (the subgenomic messenger for the coat protein). Unless RNA 3 of Tbts 7 has a second ts mutation at a site not occurring in RNA 4, it may be concluded from the above finding that RNA 4 does not replicate autonomously.
苜蓿花叶病毒的一个突变体（Tbts7），其外壳蛋白在30摄氏度时无法激活病毒基因组（RNA 1、2和3，它们的感染性需要一些外壳蛋白），通过在接种物中添加野生型RNA 3（包含外壳蛋白顺反子的基因组部分），但不添加野生型RNA 4（外壳蛋白的亚基因组信使），可在该温度下挽救该突变体。除非Tbts 7的RNA 3在RNA 4中未出现的位点有第二个温度敏感突变，否则从上述发现可以得出结论，RNA 4不能自主复制。
