Trucco Verónica, de Breuil Soledad, Bejerman Nicolás, Lenardon Sergio, Giolitti Fabián
Instituto de Patología Vegetal (IPAVE), Centro de Investigaciones Agropecuarias (CIAP), Instituto Nacional de Tecnología Agropecuaria (INTA), Camino 60 cuadras Km. 5.5, X5020ICA, Córdoba, Argentina.
Virus Genes. 2014 Jun;48(3):562-5. doi: 10.1007/s11262-014-1045-0. Epub 2014 Feb 8.
The complete nucleotide sequence of an Alfalfa mosaic virus (AMV) isolate infecting alfalfa (Medicago sativa L.) in Argentina, AMV-Arg, was determined. The virus genome has the typical organization described for AMV, and comprises 3,643, 2,593, and 2,038 nucleotides for RNA1, 2 and 3, respectively. The whole genome sequence and each encoding region were compared with those of other four isolates that have been completely sequenced from China, Italy, Spain and USA. The nucleotide identity percentages ranged from 95.9 to 99.1 % for the three RNAs and from 93.7 to 99 % for the protein 1 (P1), protein 2 (P2), movement protein and coat protein (CP) encoding regions, whereas the amino acid identity percentages of these proteins ranged from 93.4 to 99.5 %, the lowest value corresponding to P2. CP sequences of AMV-Arg were compared with those of other 25 available isolates, and the phylogenetic analysis based on the CP gene was carried out. The highest percentage of nucleotide sequence identity of the CP gene was 98.3 % with a Chinese isolate and 98.6 % at the amino acid level with four isolates, two from Italy, one from Brazil and the remaining one from China. The phylogenetic analysis showed that AMV-Arg is closely related to subgroup I of AMV isolates. To our knowledge, this is the first report of a complete nucleotide sequence of AMV from South America and the first worldwide report of complete nucleotide sequence of AMV isolated from alfalfa as natural host.
测定了在阿根廷感染紫花苜蓿（Medicago sativa L.）的一种苜蓿花叶病毒（AMV）分离株AMV-Arg的完整核苷酸序列。该病毒基因组具有AMV所描述的典型结构，RNA1、RNA2和RNA3分别由3643、2593和2038个核苷酸组成。将整个基因组序列和每个编码区域与已从中国、意大利、西班牙和美国完全测序的其他四个分离株进行了比较。三种RNA的核苷酸同一性百分比范围为95.9%至99.1%，蛋白质1（P1）、蛋白质2（P2）、运动蛋白和外壳蛋白（CP）编码区域的核苷酸同一性百分比范围为93.7%至99%，而这些蛋白质的氨基酸同一性百分比范围为93.4%至99.5%，最低值对应于P2。将AMV-Arg的CP序列与其他25个可用分离株的序列进行了比较，并基于CP基因进行了系统发育分析。CP基因核苷酸序列同一性的最高百分比与一个中国分离株为98.3%，在氨基酸水平上与四个分离株为98.6%，其中两个来自意大利，一个来自巴西，另一个来自中国。系统发育分析表明，AMV-Arg与AMV分离株的I亚组密切相关。据我们所知，这是南美洲AMV完整核苷酸序列的首次报道，也是从紫花苜蓿作为天然宿主分离的AMV完整核苷酸序列的首次全球报道。
